Anti-Diphtheria Toxin antibody [11D9] (ab53827)
Key features and details
- Mouse monoclonal [11D9] to Diphtheria Toxin
- Suitable for: Conjugation, WB, ELISA
- Isotype: IgG1
Overview
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Product name
Anti-Diphtheria Toxin antibody [11D9]
See all Diphtheria Toxin primary antibodies -
Description
Mouse monoclonal [11D9] to Diphtheria Toxin -
Host species
Mouse -
Specificity
ab53827 reacts primarily with Diphtheria toxin with a low cross reactivity with Diphtheria toxoid (formaldehyde-inactivated Diphtheria toxin). -
Tested applications
Suitable for: Conjugation, WB, ELISAmore details -
Species reactivity
Reacts with: Corynebacterium diphtheriae -
Immunogen
Chemical/ Small Molecule corresponding to Diphtheria Toxin. Diphtheria toxoid (formaldehyde inactivated Diphtheria toxin).
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General notes
For a recombinant antibody to Diphtheria Toxin, please see - ab209329.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituents: 2.9% Sodium chloride, 0.0268% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
11D9 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Conjugation |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 63 kDa (predicted molecular weight: 58 kDa).
63kDa band corresponds to the proenzyme. |
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ELISA |
Use at an assay dependent concentration.
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Notes |
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Conjugation
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 63 kDa (predicted molecular weight: 58 kDa). 63kDa band corresponds to the proenzyme. |
ELISA
Use at an assay dependent concentration. |
Target
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Relevance
Corynebacterium diphtheriae is a gram-positive, nonmotile bacteria found in soil and animal feces. C. diphteriae infect the epithelial cells of the upper respiratory tract from where they produce and secrete a potent toxin. This toxin is absorbed and disseminated through lymph channels and blood to the susceptible tissues of the body. -
Cellular localization
Secreted -
Alternative names
- Corynebacterium diphtheriae toxoid antibody
- Diphtheria Toxin antibody
- tox antibody
Datasheets and documents
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Datasheet download
References (2)
ab53827 has been referenced in 2 publications.
- Hickey JM et al. Analytical Comparability Assessments of 5 Recombinant CRM197 Proteins From Different Manufacturers and Expression Systems. J Pharm Sci 107:1806-1819 (2018). PubMed: 29526446
- Jalah R et al. Efficacy, but not antibody titer or affinity, of a heroin hapten conjugate vaccine correlates with increasing hapten densities on tetanus toxoid, but not on CRM197 carriers. Bioconjug Chem 26:1041-53 (2015). ELISA ; Mouse . PubMed: 25970207