Overview

  • Product nameAnti-DIRAS1 antibody
    See all DIRAS1 primary antibodies
  • Description
    Rabbit polyclonal to DIRAS1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Cat, Dog, Pig, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (PEQSNDYRVV VFGAGGVGKS SLVLRFVKGT FRDTYIPTIE DTYRQVISCD) of Human DIRAS1 (NP_660156).

  • Positive control
    • 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab85018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceThe three human RAS genes encode highly related 188 to 189 amino acid proteins, designated H-Ras, N-Ras and K-Ras4A and K-Ras4B (the two K-Ras proteins arise from alternative gene splicing). Ras proteins function as binary molecular switches that control intracellular signaling networks. Ras-regulated signal pathways control such processes as actin cytoskeletal integrity, proliferation, differentiation, cell adhesion, apoptosis, and cell migration. Ras and ras-related proteins are often deregulated in cancers, leading to increased invasion and metastasis, and decreased apoptosis. DIRAS1 displays low GTPase activity and exists predominantly in the GTP-bound form. It is highly expressed in heart and brain.
  • Cellular localizationCell membrane; Lipid-anchor; Cytoplasmic side
  • Database links
  • Alternative names
    • Di Ras1 antibody
    • DIRAS family GTP binding RAS like 1 antibody
    • Distinct subgroup of the Ras family member 1 antibody
    • FLJ42681 antibody
    • GBTS1 antibody
    • GTP binding protein Di Ras1 antibody
    • Ras related inhibitor of cell growth antibody
    • RIG antibody
    • Rig protein antibody
    • Small GTP binding tumor suppressor 1 antibody
    see all

Anti-DIRAS1 antibody images

  • Anti-DIRAS1 antibody (ab85018) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 22 kDa
    Observed band size : 22 kDa
    Additional bands at : 67 kDa. We are unsure as to the identity of these extra bands.

References for Anti-DIRAS1 antibody (ab85018)

ab85018 has not yet been referenced specifically in any publications.

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