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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: PBS, pH 7.2
Concentration information loading...
Protein A purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 22 kDa).
Detection Antibody Pair.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Anti-DIRAS2 antibody images
Western blot - DIRAS2 antibody (ab67430)
All lanes :
Anti-DIRAS2 antibody (ab67430) at 1/500 dilution
Lane 1 :
DIRAS2 transfected 293T cell lysate
Lane 2 :
Non-transfected 293T cell lysate
Lysates/proteins at 25 µg/ml per lane.
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size :
Observed band size :
24 kDa (
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIRAS2 antibody (ab67430) Image courtesy of an anonymous Abreview.
ab67430 staining DIRAS2 in rat brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in paraformaldehyde, a heat mediated antigen retrieval step was performed using Tris-EDTA pH9, samples were then blocked using 5% serum and then incubated with ab67430 at a 1/100 dilution for 1 hour at 25°C. The secondary used was an undiluted biotin conjugated goat polyclonal.
References for Anti-DIRAS2 antibody (ab67430)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"