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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: None
Constituents: PBS, pH 7.2
Concentration information loading...
Purity Protein A purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes WB: 1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 22 kDa).
Detection Antibody Pair.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Relevance DIRAS2 displays low GTPase activity and exist predominantly in the GTP-bound form. It is highly expressed in the brain.
Cellular localization Cell membrane; Lipid-anchor; Cytoplasmic side
Di Ras2 antibody
DIRAS family, GTP binding RAS like 2 antibody
Anti-DIRAS2 antibody images
Western blot - DIRAS2 antibody (ab67430)
All lanes : Anti-DIRAS2 antibody (ab67430) at 1/500 dilution Lane 1 : DIRAS2 transfected 293T cell lysate Lane 2 : Non-transfected 293T cell lysate Lysates/proteins at 25 µg/ml per lane. Secondary Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution Predicted band size : 22 kDa Observed band size : 24 kDa ( why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIRAS2 antibody (ab67430) Image courtesy of an anonymous Abreview.
ab67430 staining DIRAS2 in rat brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed in paraformaldehyde, a heat mediated antigen retrieval step was performed using Tris-EDTA pH9, samples were then blocked using 5% serum and then incubated with ab67430 at a 1/100 dilution for 1 hour at 25°C. The secondary used was an undiluted biotin conjugated goat polyclonal.
References for Anti-DIRAS2 antibody (ab67430)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"