The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 1-10µg/mg of lysate.
WB: 1/1000 - 1/10000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation (By similarity). May be involved in apoptosis signaling in the presence of the reinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation.
Expressed in various epithelial cancer cell lines, including breast, colon, prostate, pancreatic and leukemia. Expression is regulated by growth factors.
Contains 1 SAP domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Lane 1 : Nuclear extract from HeLa cells at 15 µg Lane 2 : Nuclear extract from HeLa cells at 45 µg Lane 3 : Nuclear extract from HeLa cells at 15 µg Lane 4 : Nuclear extract from HeLa cells at 45 µg
Developed using the ECL technique
Predicted band size : 132 kDa Observed band size : 132 kDa
Exposure time : 1 minute
Immunoprecipitation - DIS antibody (ab70245)
Detection of Human CCAR1 by Immunoprecipitation using
Nuclear extract (100 µg) from HeLa cells and
Affinity purified rabbit anti-CCAR1 ab70245 at 1 µg/100 µg Nuclear extract.
Chemiluminescence with exposure times of approximately 3 seconds.