Overview

  • Product nameAnti-Dis3 antibody
  • Description
    Rabbit polyclonal to Dis3
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Schizosaccharomyces pombe
  • Immunogen

    Recombinant fragment corresponding to Schizosaccharomyces pombe Dis3.
    Database link: P37202

  • Positive control
    • S. pombe cell lysates transformed with vector or plasmids carrying truncated genes; S.pombe cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab64289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/300. Predicted molecular weight: 110 kDa.
ICC/IF Use at an assay dependent concentration.

Target

  • RelevanceDis 3 is a catalytic component of the exosome 3'->5' exoribonuclease complex required for the 3'-processing of the 7S pre-RNA to the mature 5.8S rRNA and for mRNA decay. Has a 3'-5' exonuclease activity. Implicated in mitotic control. Essential for cell division and spore germination. May be involved in regulating protein dephosphorylation during mitosis.
  • Cellular localizationCytoplasmic and Nuclear
  • Database links
    • Alternative names
      • 3'-5' exoribonuclease subunit Dis3 antibody
      • Chromosome disjunction protein 3 antibody
      • Exosome complex exonuclease dis3 antibody
      • Mitotic control protein dis3 antibody
      • Ribosomal RNA processing protein 44 antibody
      • rrp44 antibody
      see all

    Anti-Dis3 antibody images

    • Immunfluorescent analysis of S. pombe cells labeling Dis3 with ab64289 (right panel). DAPI stain for chromosomal DNA (left panel).

    • All lanes : Anti-Dis3 antibody (ab64289) at 1/100 dilution

      Lane 1 : S. pombe cell lysate transformed with the vector
      Lane 2 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (172)
      Lane 3 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (A)
      Lane 4 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (B)
      Lane 5 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (C)
      Lane 6 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (E)


      Predicted band size : 110 kDa

      Polypeptides of expected molecular masses were detected.

    References for Anti-Dis3 antibody (ab64289)

    This product has been referenced in:
    • Kinoshita N  et al. The fission yeast dis3+ gene encodes a 110-kDa essential protein implicated in mitotic control. Mol Cell Biol 11:5839-47 (1991). Read more (PubMed: 1944266) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab64289.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"