Overview

  • Product name
  • Description
    Rabbit polyclonal to DIS3L2
  • Tested applications
    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: DLVVVKLLPE EHWKVVKPES NDKETEAAYE SDIPEELCGH HLPQQSLKSY, corresponding to amino acids 109-158 of Human DIS3L2

  • Positive control
    • Jurkat Cell Lysate or Human Myocardial cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 8 µg/ml.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 69 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

Target

  • Function
    Ribonuclease that plays a critical role in RNA metabolism. It is essential for correct mitosis, and negatively regulates cell proliferation.
  • Involvement in disease
    Defects in DIS3L2 are the cause of Perlman syndrome (PRLMNS) [MIM:267000]. An autosomal recessive congenital overgrowth syndrome. Affected children are large at birth, are hypotonic, and show organomegaly, characteristic facial dysmorphisms (inverted V-shaped upper lip, prominent forehead, deep-set eyes, broad and flat nasal bridge, and low-set ears), renal anomalies (nephromegaly and hydronephrosis), frequent neurodevelopmental delay, and high neonatal mortality. Perlman syndrome is associated with a high risk of Wilms tumor. Histologic examination of the kidneys in affected children shows frequent nephroblastomatosis, which is a precursor lesion for Wilms tumor.
  • Sequence similarities
    Belongs to the RNR ribonuclease family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • DI3L2_HUMAN antibody
    • DIS3 L2 antibody
    • DIS3 mitotic control homolog (S. cerevisiae) like 2 antibody
    • DIS3 mitotic control homolog like 2 antibody
    • DIS3-like exonuclease 2 antibody
    • Dis3l2 antibody
    • FLJ36974 antibody
    • MGC42174 antibody
    see all

Images

  • All lanes : Anti-DIS3L2 antibody (ab50848) at 1.25 µg/ml

    Lane 1 : MW marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 69 kDa
    Observed band size : 63 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling DIS3L2 with ab50848 at 4-8µg/ml. Arrows indicate positively stained human myocardial cells. Magnification: 400X.

References

ab50848 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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