• Product nameAnti-DIS3L2 antibody
    See all DIS3L2 primary antibodies
  • Description
    Rabbit polyclonal to DIS3L2
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: DLVVVKLLPE EHWKVVKPES NDKETEAAYE SDIPEELCGH HLPQQSLKSY, corresponding to amino acids 109-158 of Human DIS3L2

  • Positive control
    • Jurkat Cell Lysate or Human Myocardial cells


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab50848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 8 µg/ml.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 69 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.


  • FunctionRibonuclease that plays a critical role in RNA metabolism. It is essential for correct mitosis, and negatively regulates cell proliferation.
  • Involvement in diseaseDefects in DIS3L2 are the cause of Perlman syndrome (PRLMNS) [MIM:267000]. An autosomal recessive congenital overgrowth syndrome. Affected children are large at birth, are hypotonic, and show organomegaly, characteristic facial dysmorphisms (inverted V-shaped upper lip, prominent forehead, deep-set eyes, broad and flat nasal bridge, and low-set ears), renal anomalies (nephromegaly and hydronephrosis), frequent neurodevelopmental delay, and high neonatal mortality. Perlman syndrome is associated with a high risk of Wilms tumor. Histologic examination of the kidneys in affected children shows frequent nephroblastomatosis, which is a precursor lesion for Wilms tumor.
  • Sequence similaritiesBelongs to the RNR ribonuclease family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • DI3L2_HUMAN antibody
    • DIS3 L2 antibody
    • DIS3 mitotic control homolog (S. cerevisiae) like 2 antibody
    • DIS3 mitotic control homolog like 2 antibody
    • DIS3-like exonuclease 2 antibody
    • Dis3l2 antibody
    • FLJ36974 antibody
    • MGC42174 antibody
    see all

Anti-DIS3L2 antibody images

  • All lanes : Anti-DIS3L2 antibody (ab50848) at 1.25 µg/ml

    Lane 1 : MW marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 69 kDa
    Observed band size : 63 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling DIS3L2 with ab50848 at 4-8µg/ml. Arrows indicate positively stained human myocardial cells. Magnification: 400X.

References for Anti-DIS3L2 antibody (ab50848)

ab50848 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab50848.
Please use the links above to contact us or submit feedback about this product.