Note: dilute ab22618 to working strength using 50mM PBS (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat antirabbit IgG linker antibody is to be used).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionParticipates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes.
Sequence similaritiesBelongs to the DSH family. Contains 1 DEP domain. Contains 1 DIX domain. Contains 1 PDZ (DHR) domain.
Cellular localizationCell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta.
Dishevelled 2 (homologous to Drosophila dsh) antibody
Dishevelled dsh homolog 2 antibody
dishevelled segment polarity protein 2 antibody
DSH homolog 2 antibody
DVL 2 antibody
Segment polarity protein dishevelled homolog DVL 2 antibody
Segment polarity protein dishevelled homolog DVL-2 antibody
Segment polarity protein dishevelled homolog DVL2 antibody
Anti-Dishevelled 2 antibody images
Immunohistochemistry (Frozen sections) - Dishevelled 2 antibody (ab22618)Image courtesy of an anonymous Abreview.
ab22618 staining Dishevelled 2 in murine rib cartilage by Immunohistochemistry (Frozen sections). Tissue was fixed with paraformaldehyde and permeabilized using 0.1% Triton. Samples were then blocked with 20% serum for 1 hour at 19°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An Alexa Fluor® conjugated donkey anti-rabbit polyclonal was used as secondary antibody at a 1/200 dilution. Counterstain DAPI (blue).