Anti-Dishevelled 2 antibody (ab22618)

Overview

  • Product name
    Anti-Dishevelled 2 antibody
    See all Dishevelled 2 primary antibodies
  • Description
    Rabbit polyclonal to Dishevelled 2
  • Specificity
    ab22618 recognises dishevelled 2. It doesn't cross-react with dishevelled 1 or dishevelled 3.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    RGGEASGTSDGGP(C)

    conjugated to KLH by a MBS linker, corresponding to amino acids 633/645 of Human Dishevelled 2.

  • Positive control
    • Dishevelled 2 transfected HEK293 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 10mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    ab22618 is a rabbit antiserum partially purified by caprylic acid and ammonium sulphate precipitation.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/1000. Predicted molecular weight: 18 kDa.

    Note: dilute ab22618 to working strength using 50mM PBS (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat antirabbit IgG linker antibody is to be used).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes.
    • Sequence similarities
      Belongs to the DSH family.
      Contains 1 DEP domain.
      Contains 1 DIX domain.
      Contains 1 PDZ (DHR) domain.
    • Domain
      The DIX domain mediates homooligomerization.
    • Cellular localization
      Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta.
    • Information by UniProt
    • Database links
    • Alternative names
      • Dishevelled 2 (homologous to Drosophila dsh) antibody
      • Dishevelled dsh homolog 2 antibody
      • dishevelled segment polarity protein 2 antibody
      • Dishevelled-2 antibody
      • Dishevelled2 antibody
      • DSH homolog 2 antibody
      • DVL 2 antibody
      • Dvl2 antibody
      • DVL2_HUMAN antibody
      • Segment polarity protein dishevelled homolog DVL 2 antibody
      • Segment polarity protein dishevelled homolog DVL-2 antibody
      • Segment polarity protein dishevelled homolog DVL2 antibody
      see all

    Images

    • ab22618 staining Dishevelled 2 in murine rib cartilage by Immunohistochemistry (Frozen sections). Tissue was fixed with paraformaldehyde and permeabilized using 0.1% Triton. Samples were then blocked with 20% serum for 1 hour at 19°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An Alexa Fluor® conjugated donkey anti-rabbit polyclonal was used as secondary antibody at a 1/200 dilution. Counterstain DAPI (blue).

      See Abreview

    References

    ab22618 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Rib cartilage)
    Specification
    Rib cartilage
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 19°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 25 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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