The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: Use at a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 78 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay play a role in the signal transduction pathway mediated by multiple Wnt genes.
Sequence similaritiesBelongs to the DSH family. Contains 1 DEP domain. Contains 1 DIX domain. Contains 1 PDZ (DHR) domain.
Post-translational modificationsUbiquitinated. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains.