Anti-Dishevelled 3 antibody - N-terminal (ab152161)
Key features and details
- Rabbit polyclonal to Dishevelled 3 - N-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Dishevelled 3 antibody - N-terminal
See all Dishevelled 3 primary antibodies -
Description
Rabbit polyclonal to Dishevelled 3 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment corresponding to a region within N-terminal amino acids 1-166 of Human Dishevelled 3 (Uniprot ID: Q92997).
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Positive control
- Human SW480 xenograft tissue; HCT116 cells; HCT116 whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab152161 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000 - 1/20000. Predicted molecular weight: 78 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/5000 - 1/20000. Predicted molecular weight: 78 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0). |
ICC/IF
1/100 - 1/1000. |
Target
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Function
May play a role in the signal transduction pathway mediated by multiple Wnt genes. -
Sequence similarities
Belongs to the DSH family.
Contains 1 DEP domain.
Contains 1 DIX domain.
Contains 1 PDZ (DHR) domain. -
Post-translational
modificationsUbiquitinated. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1857 Human
- Entrez Gene: 13544 Mouse
- Omim: 601368 Human
- SwissProt: Q92997 Human
- SwissProt: Q61062 Mouse
- Unigene: 388116 Human
- Unigene: 247259 Mouse
- Unigene: 272443 Mouse
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Alternative names
- Dishevelled 3 (homologous to Drosophila dsh) antibody
- Dishevelled 3 antibody
- Dishevelled 3, dsh homolog (Drosophila) antibody
see all
Images
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Anti-Dishevelled 3 antibody - N-terminal (ab152161) at 1/10000 dilution + HCT116 whole cell lysate at 30 µg
Predicted band size: 78 kDa
7.5% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human SW480 xenograft tissue, staining Dishevelled 3 using ab152161 at a 1/100 dilution.
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Immunofluorescence analysis of methanol-fixed HCT116 cells, staining Dishevelled 3 (green) using ab152161 at a 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab152161 has not yet been referenced specifically in any publications.