Anti-Dishevelled 3 antibody - N-terminal (ab152161)

Overview

  • Product name
    Anti-Dishevelled 3 antibody - N-terminal
    See all Dishevelled 3 primary antibodies
  • Description
    Rabbit polyclonal to Dishevelled 3 - N-terminal
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment corresponding to a region within N-terminal amino acids 1-166 of Human Dishevelled 3 (Uniprot ID: Q92997).

  • Positive control
    • Human SW480 xenograft tissue; HCT116 cells; HCT116 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab152161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 78 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function
    May play a role in the signal transduction pathway mediated by multiple Wnt genes.
  • Sequence similarities
    Belongs to the DSH family.
    Contains 1 DEP domain.
    Contains 1 DIX domain.
    Contains 1 PDZ (DHR) domain.
  • Post-translational
    modifications
    Ubiquitinated. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dishevelled 3 (homologous to Drosophila dsh) antibody
    • Dishevelled 3 antibody
    • Dishevelled 3, dsh homolog (Drosophila) antibody
    • Dishevelled, dsh homolog 3 (Drosophila) antibody
    • Dishevelled-3 antibody
    • DSH homolog 3 antibody
    • dvl3 antibody
    • DVL3_HUMAN antibody
    • KIAA0208 antibody
    • OTTHUMP00000210777 antibody
    • OTTHUMP00000210778 antibody
    • Segment polarity protein dishevelled homolog DVL 3 antibody
    • Segment polarity protein dishevelled homolog DVL-3 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human SW480 xenograft tissue, staining Dishevelled 3 using ab152161 at a 1/100 dilution.
  • Immunofluorescence analysis of methanol-fixed HCT116 cells, staining Dishevelled 3 (green) using ab152161 at a 1/500 dilution.
  • Anti-Dishevelled 3 antibody - N-terminal (ab152161) at 1/10000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size : 78 kDa

References

ab152161 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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