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Rabbit polyclonal to Dishevelled 3 - N-terminal
HumanPredicted to work with:
Recombinant fragment corresponding to a region within N-terminal amino acids 1-166 of Human Dishevelled 3 (Uniprot ID: Q92997).
- Human SW480 xenograft tissue; HCT116 cells; HCT116 whole cell lysate.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/5000 - 1/20000. Predicted molecular weight: 78 kDa.
||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
||1/100 - 1/1000.
May play a role in the signal transduction pathway mediated by multiple Wnt genes.
Belongs to the DSH family.
Contains 1 DEP domain.
Contains 1 DIX domain.
Contains 1 PDZ (DHR) domain.
Ubiquitinated. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains.
Information by UniProt
- Dishevelled 3 (homologous to Drosophila dsh) antibody
- Dishevelled 3 antibody
- Dishevelled 3, dsh homolog (Drosophila) antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dishevelled 3 antibody - N-terminal (ab152161)
Immunohistochemical analysis of paraffin-embedded Human SW480 xenograft tissue, staining Dishevelled 3 using ab152161 at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Dishevelled 3 antibody - N-terminal (ab152161)
Immunofluorescence analysis of methanol-fixed HCT116 cells, staining Dishevelled 3 (green) using ab152161 at a 1/500 dilution.
Western blot - Anti-Dishevelled 3 antibody - N-terminal (ab152161)
Anti-Dishevelled 3 antibody - N-terminal (ab152161) at 1/10000 dilution + HCT116 whole cell lysate at 30 µg
Predicted band size: 78 kDa
7.5% SDS PAGE
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"