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Anti-Dispatched antibody (ab10142)

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Overview

Product name

Anti-Dispatched antibody
See all Dispatched products (3) ...

Description

Goat polyclonal to Dispatched

Tested applications

ICC/IF, WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat

Immunogen

Synthetic peptide: SELSGESLLIKTL, corresponding to C terminal amino acids 1512-1524 of Human Dispatched.

SELSGESLLI KTL

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Primary antibody notes

Dispatched is a novel sterol-sensing domain protein dedicated to the release of cholesterol-modified hedgehog from signaling cells.

Clonality

Polyclonal

Isotype

IgG

  • Immunocytochemistry/ Immunofluorescence - Dispatched antibody (ab10142)Immunocytochemistry/ Immunofluorescence - Dispatched antibody (ab10142) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab10142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    ICC/IF

     ICC/IF: Use at an assay ...Read more →

    ICC/IF: Use at an assay dependent dilution. (Cell membrane staining in cell line U87)

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    WB

     WB: Use at an assay depe...Read more →

    WB: Use at an assay dependent dilution. (Preliminary experiments gave no signal but low background in Human Lung and A549 extracts at up to 1µg/ml.)

Target

Function

Functions in hedgehog (Hh) signaling. Regulates the release and extracellular accumulation of cholesterol-modified hedgehog proteins and is hence required for effective production of the Hh signal.

Sequence similarities

Belongs to the dispatched family.
Contains 1 SSD (sterol-sensing) domain.

Cellular localization

Membrane.

Target information above from: UniProt accessionQ96F81 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • DISP 1 antibody
  • disp1 antibody
  • DISP1_HUMAN antibody
  • DISPA antibody
  • dispatched A antibody
  • Dispatched homolog 1 antibody
  • DKFZP434I0428 antibody
  • FLJ43740 antibody
  • MGC104180 antibody
  • MGC13130 antibody
  • MGC16796 antibody
  • Protein dispatched homolog 1 antibody
see all

Anti-Dispatched antibody images:

  Immunocytochemistry/ Immunofluorescence - Dispatched antibody (ab10142)

Immunocytochemistry/ Immunofluorescence - Dispatched antibody (ab10142)

ab10142 staining Dispatched at 2.5µg/ml in U87 cells by Immunocytochemistry/ Immunofluorescence.

References for Anti-Dispatched antibody (ab10142)

ab10142 has not yet been referenced specifically in any publications.

Publishing research using ab10142? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"