Overview

  • Product name
  • Description
    Goat polyclonal to Dispatched
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    SELSGESLLIKTL

    , corresponding to C terminal amino acids 1512-1524 of Human Dispatched.

  • General notes


    Dispatched is a novel sterol-sensing domain protein dedicated to the release of cholesterol-modified hedgehog from signaling cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody notes
    Dispatched is a novel sterol-sensing domain protein dedicated to the release of cholesterol-modified hedgehog from signaling cells.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution. Cell membrane staining in cell line U87
WB Use at an assay dependent dilution. Preliminary experiments gave no signal but low background in Human Lung and A549 extracts at up to 1µg/ml.

Target

Images

  • ab10142 staining Dispatched at 2.5µg/ml in U87 cells by Immunocytochemistry/ Immunofluorescence.

References

ab10142 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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