Overview

  • Product nameAnti-Dispatched antibody
    See all Dispatched primary antibodies
  • Description
    Goat polyclonal to Dispatched
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    SELSGESLLIKTL

    , corresponding to C terminal amino acids 1512-1524 of Human Dispatched.

  • General notes


    Dispatched is a novel sterol-sensing domain protein dedicated to the release of cholesterol-modified hedgehog from signaling cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody notesDispatched is a novel sterol-sensing domain protein dedicated to the release of cholesterol-modified hedgehog from signaling cells.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution. Cell membrane staining in cell line U87
WB Use at an assay dependent dilution. Preliminary experiments gave no signal but low background in Human Lung and A549 extracts at up to 1µg/ml.

Target

Anti-Dispatched antibody images

  • ab10142 staining Dispatched at 2.5µg/ml in U87 cells by Immunocytochemistry/ Immunofluorescence.

References for Anti-Dispatched antibody (ab10142)

ab10142 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"