The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Cell membrane staining in cell line U87
Use at an assay dependent dilution. Preliminary experiments gave no signal but low background in Human Lung and A549 extracts at up to 1µg/ml.
FunctionFunctions in hedgehog (Hh) signaling. Regulates the release and extracellular accumulation of cholesterol-modified hedgehog proteins and is hence required for effective production of the Hh signal.
Sequence similaritiesBelongs to the dispatched family. Contains 1 SSD (sterol-sensing) domain.