Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Neuroscience >> Neurology process >> Neural Signal Transduction
Overview
Product name
Anti-Dispatched antibody
See all Dispatched products (3) ...
Description
Goat polyclonal to Dispatched
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide: SELSGESLLIKTL, corresponding to C terminal amino acids 1512-1524 of Human Dispatched.
SELSGESLLI KTL
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Primary antibody notes
Dispatched is a novel sterol-sensing domain protein dedicated to the release of cholesterol-modified hedgehog from signaling cells.
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neural Signal Transduction
-
Immunocytochemistry/ Immunofluorescence - Dispatched antibody (ab10142)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10142 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution. (Cell membrane staining in cell line U87)
WB: Use at an assay dependent dilution. (Preliminary experiments gave no signal but low background in Human Lung and A549 extracts at up to 1µg/ml.)
Target
Function
Functions in hedgehog (Hh) signaling. Regulates the release and extracellular accumulation of cholesterol-modified hedgehog proteins and is hence required for effective production of the Hh signal.
Sequence similarities
Belongs to the dispatched family.
Contains 1 SSD (sterol-sensing) domain.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ96F81
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DISP 1 antibody
- disp1 antibody
- DISP1_HUMAN antibody
see all
Database links
- Entrez Gene: 84976 Human
- Entrez Gene: 68897 Mouse
- Entrez Gene: 289338 Rat
- Omim: 607502 Human
- SwissProt: Q96F81 Human
- SwissProt: Q3TDN0 Mouse
- Unigene: 528817 Human
- Unigene: 358721 Mouse
see all
Anti-Dispatched antibody images:
Immunocytochemistry/ Immunofluorescence - Dispatched antibody (ab10142)
ab10142 staining Dispatched at 2.5µg/ml in U87 cells by Immunocytochemistry/ Immunofluorescence.
References for Anti-Dispatched antibody (ab10142)
ab10142 has not yet been referenced specifically in any publications.
Publishing research using ab10142? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"