Overview

  • Product nameAnti-DIXDC1 antibody
    See all DIXDC1 primary antibodies
  • Description
    Mouse polyclonal to DIXDC1
  • SpecificityAntibody reactive against mammalian transfected lysate.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-472 of Human DIXDC1

  • Positive control
    • Transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 78 kDa).
    Detection pair

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPositive effector of the Wnt signaling pathway; activates WNT3A signaling via DVL2. Regulates JNK activation by AXIN1 and DVL2.
    • Tissue specificityUbiquitously expressed with higher expression in cardiac and skeletal muscles.
    • Sequence similaritiesBelongs to the DIXDC1 family.
      Contains 1 CH (calponin-homology) domain.
      Contains 1 DIX domain.
    • DomainThe coiled-coil domain mediates interaction with MAP3K4 and inhibition of AXIN1-mediated JNK activation through MAP3K4.
      The DIX domain mediates interaction with AXIN1 and inhibition of AXIN1-mediated JNK activation through MAP3K1. Mediates interaction with DVL2; this interaction is required for activation of Wnt signaling.
    • Post-translational
      modifications
      Phosphorylated on tyrosine and serine residues.
      Polyubiquitinated, leading to its proteasomal degradation. WNT3A signaling increases DIXDC1 protein levels by inhibiting its ubiquitination and subsequent degradation.
    • Cellular localizationCytoplasm; Cell junction > focal adhesion. Associated with actin stress fiber at the filament ends and Cytoplasm > cytosol. Colocalizes with gamma-tubulin at the centrosome, both during interphase and mitosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • CCD1 antibody
      • Ccd1Aalpha1L antibody
      • Ccd1Abeta1L antibody
      • Ccd1BalphaL antibody
      • Ccd1BbetaL antibody
      • Ccd1CL antibody
      • Coiled coil DIX1 antibody
      • Coiled coil protein DIX1 antibody
      • Coiled-coil protein DIX1 antibody
      • Coiled-coil-DIX1 antibody
      • DIX domain containing 1 antibody
      • DIX domain containing protein 1 antibody
      • DIX domain-containing protein 1 antibody
      • DIXC1_HUMAN antibody
      • DIXDC1 antibody
      • Dixin antibody
      • KIAA1735 antibody
      see all

    Anti-DIXDC1 antibody images

    • All lanes : Anti-DIXDC1 antibody (ab67763) at 1/500 dilution

      Lane 1 : DIXDC1 transfected lysate
      Lane 2 : Non transfected lysate.

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

      Predicted band size : 78 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)

    References for Anti-DIXDC1 antibody (ab67763)

    ab67763 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"