Overview

  • Product nameAnti-DIXDC1 antibody
    See all DIXDC1 primary antibodies
  • Description
    Goat polyclonal to DIXDC1
  • Specificityab99283 is expected to recognize both isoforms (NP_001033043.1; NP_219493.1).
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    C-DVSYHQVDLERE

    , corresponding to internal sequence amino acids 239-250 of Human DIXDC1 (NP_219493.1).

  • Positive control
    • Human Heart and Skeletal muscle lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab99283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 54 kDa).

Target

  • FunctionPositive effector of the Wnt signaling pathway; activates WNT3A signaling via DVL2. Regulates JNK activation by AXIN1 and DVL2.
  • Tissue specificityUbiquitously expressed with higher expression in cardiac and skeletal muscles.
  • Sequence similaritiesBelongs to the DIXDC1 family.
    Contains 1 CH (calponin-homology) domain.
    Contains 1 DIX domain.
  • DomainThe coiled-coil domain mediates interaction with MAP3K4 and inhibition of AXIN1-mediated JNK activation through MAP3K4.
    The DIX domain mediates interaction with AXIN1 and inhibition of AXIN1-mediated JNK activation through MAP3K1. Mediates interaction with DVL2; this interaction is required for activation of Wnt signaling.
  • Post-translational
    modifications
    Phosphorylated on tyrosine and serine residues.
    Polyubiquitinated, leading to its proteasomal degradation. WNT3A signaling increases DIXDC1 protein levels by inhibiting its ubiquitination and subsequent degradation.
  • Cellular localizationCytoplasm; Cell junction > focal adhesion. Associated with actin stress fiber at the filament ends and Cytoplasm > cytosol. Colocalizes with gamma-tubulin at the centrosome, both during interphase and mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCD1 antibody
    • Ccd1Aalpha1L antibody
    • Ccd1Abeta1L antibody
    • Ccd1BalphaL antibody
    • Ccd1BbetaL antibody
    • Ccd1CL antibody
    • Coiled coil DIX1 antibody
    • Coiled coil protein DIX1 antibody
    • Coiled-coil protein DIX1 antibody
    • Coiled-coil-DIX1 antibody
    • DIX domain containing 1 antibody
    • DIX domain containing protein 1 antibody
    • DIX domain-containing protein 1 antibody
    • DIXC1_HUMAN antibody
    • DIXDC1 antibody
    • Dixin antibody
    • KIAA1735 antibody
    see all

Anti-DIXDC1 antibody images

  • Anti-DIXDC1 antibody (ab99283) at 0.5 µg/ml + Human skeletal muscle lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 54 kDa

References for Anti-DIXDC1 antibody (ab99283)

ab99283 has not yet been referenced specifically in any publications.

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