Overview

  • Product name
    Anti-DjC8 antibody
    See all DjC8 primary antibodies
  • Description
    Rabbit polyclonal to DjC8
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Orangutan, Platypus
  • Immunogen

    Synthetic peptide corresponding to a region between residue 203 and 253 of Human DjC8 (NP_055095.2).

  • Positive control
    • Whole cell lysate from HeLa cells and 293T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab86138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 30 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

Images

  • Western blot - Anti-DjC8 antibody (ab86138)
    Western blot - Anti-DjC8 antibody (ab86138)
    All lanes : Anti-DjC8 antibody (ab86138) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    Developed using the ECL technique.

    Predicted band size: 30 kDa
    Observed band size: 36 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 18 kDa, 48 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-DjC8 antibody (ab86138)
    Immunoprecipitation - Anti-DjC8 antibody (ab86138)
    Detection of DjC8 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86138 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 seconds.

References

ab86138 has not yet been referenced specifically in any publications.

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