- Datasheet
- Specific References
- Protocols
Overview
- Product nameAnti-DjC8 antibody
See all DjC8 primary antibodies - DescriptionRabbit polyclonal to DjC8
- Tested applicationsSuitable for: WB, IPmore details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Orangutan, Platypus
- Immunogen
Synthetic peptide corresponding to a region between residue 203 and 253 of Human DjC8 (NP_055095.2).
- Positive control
- Whole cell lysate from HeLa cells and 293T cells.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... - PurityImmunogen affinity purified
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
- Positive Controls
Applications
Our Abpromise guarantee covers the use of ab86138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 30 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. |
Target
- Sequence similaritiesContains 1 J domain.
- Information by UniProt
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Database links
- Entrez Gene: 22826 Human
- Entrez Gene: 68598 Mouse
- Entrez Gene: 313035 Rat
- SwissProt: O75937 Human
- SwissProt: Q6NZB0 Mouse
- SwissProt: Q642C0 Rat
- Unigene: 433540 Human
- Unigene: 29685 Mouse
see all -
Alternative names
- DnaJ (Hsp40) homolog subfamily C member 8 antibody
- DnaJ homolog subfamily C member 8 antibody
- DNAJC 8 antibody
see all
Anti-DjC8 antibody images
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Western blot - DjC8 antibody (ab86138)All lanes : Anti-DjC8 antibody (ab86138) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Developed using the ECL technique
Predicted band size : 30 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
Additional bands at : 18 kDa,48 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes -
Immunoprecipitation - DjC8 antibody (ab86138)Detection of DjC8 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86138 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 seconds.
References for Anti-DjC8 antibody (ab86138)
ab86138 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"