Anti-DKC1/Dyskerin antibody (ab93777)
Key features and details
- Rabbit polyclonal to DKC1/Dyskerin
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-DKC1/Dyskerin antibody
See all DKC1/Dyskerin primary antibodies -
Description
Rabbit polyclonal to DKC1/Dyskerin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Drosophila melanogaster, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis -
Immunogen
Synthetic peptide corresponding to Human DKC1/Dyskerin aa 340-390.
Database link: NP_001354.1 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93777 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 58 kDa.
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IP |
Use at 5-15 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 58 kDa. |
IP
Use at 5-15 µg/mg of lysate. |
Target
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Function
Isoform 1: Required for ribosome biogenesis and telomere maintenance. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
Isoform 3: Promotes cell to cell and cell to substratum adhesion, increases the cell proliferation rate and leads to cytokeratin hyper-expression (when overexpressed in HeLa cells). -
Tissue specificity
Ubiquitously expressed. -
Involvement in disease
Defects in DKC1 are a cause of dyskeratosis congenita X-linked recessive (XDKC) [MIM:305000]. XDKC is a rare, progressive bone marrow failure syndrome characterized by the triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.
Defects in DKC1 are the cause of Hoyeraal-Hreidarsson syndrome (HHS) [MIM:300240]. HHS is a multisystem disorder affecting males and is characterized by aplastic anemia, immunodeficiency, microcephaly, cerebellar hypoplasia, and growth retardation. -
Sequence similarities
Belongs to the pseudouridine synthase TruB family.
Contains 1 PUA domain. -
Cellular localization
Cytoplasm and Nucleus > nucleolus. Nucleus > Cajal body. Also localized to Cajal bodies. - Information by UniProt
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Database links
- Entrez Gene: 37873 Drosophila melanogaster
- Entrez Gene: 1736 Human
- Entrez Gene: 245474 Mouse
- Entrez Gene: 170944 Rat
- Omim: 300126 Human
- SwissProt: O44081 Drosophila melanogaster
- SwissProt: O60832 Human
- SwissProt: Q9ESX5 Mouse
see all -
Alternative names
- CBF5 antibody
- CBF5 homolog antibody
- Cbf5p homolog antibody
see all
Images
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All lanes : Anti-DKC1/Dyskerin antibody (ab93777) at 0.04 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 58 kDa
Exposure time: 3 minutes -
Detection of Human DKC1/Dyskerin by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded)using ab93777 at 10 ug/mg lysate (LANE 1). DKC1/Dyskerin was also immunoprecipitated by an antibody which recognizes a downstream epitope (LANE 2). Lane 3 represents IgG Ip control. subsequent WB detection was performed using 1 ug/ml ab93777. Detection: Chemiluminescence with exposure times of 10 seconds.
All lanes : Anti-DKC1/Dyskerin antibody (ab93777) at 1 µg/ml
Lane 1 : HeLa cell lysate immunoprecipitated with ab 93777 at 10ug/mg lysate.
Lane 2 : HeLa cell lysate immunoprecipitated with anti-DKC1/Dyskerinantibody at 10ug/mg lysate.
Lane 3 : HeLa cell lysate immunoprecipitated with control IgG at 10ug/mg lysate.
Lysates/proteins at 200 µg per lane.
Developed using the ECL technique.
Exposure time: 10 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab93777 has been referenced in 3 publications.
- Yang Z et al. LncRNA MEG3 inhibits non-small cell lung cancer via interaction with DKC1 protein. Oncol Lett 20:2183-2190 (2020). PubMed: 32782535
- Yao RW et al. Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus. Mol Cell 76:767-783.e11 (2019). PubMed: 31540874
- Xing YH et al. SLERT Regulates DDX21 Rings Associated with Pol I Transcription. Cell 169:664-678.e16 (2017). PubMed: 28475895