Overview

  • Product nameAnti-DKC1 antibody
    See all DKC1 primary antibodies
  • Description
    Rabbit polyclonal to DKC1
  • Tested applicationsSuitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Drosophila melanogaster, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 340-390 of Human DKC1 (NP_001354.1)

  • Positive control
    • HeLa and 293T cell lysate This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab93777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
IP
  • Application notesIP: Use at 5-15µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 58 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionIsoform 1: Required for ribosome biogenesis and telomere maintenance. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
      Isoform 3: Promotes cell to cell and cell to substratum adhesion, increases the cell proliferation rate and leads to cytokeratin hyper-expression (when overexpressed in HeLa cells).
    • Tissue specificityUbiquitously expressed.
    • Involvement in diseaseDefects in DKC1 are a cause of dyskeratosis congenita X-linked recessive (XDKC) [MIM:305000]. XDKC is a rare, progressive bone marrow failure syndrome characterized by the triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.
      Defects in DKC1 are the cause of Hoyeraal-Hreidarsson syndrome (HHS) [MIM:300240]. HHS is a multisystem disorder affecting males and is characterized by aplastic anemia, immunodeficiency, microcephaly, cerebellar hypoplasia, and growth retardation.
    • Sequence similaritiesBelongs to the pseudouridine synthase TruB family.
      Contains 1 PUA domain.
    • Cellular localizationCytoplasm and Nucleus > nucleolus. Nucleus > Cajal body. Also localized to Cajal bodies.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • CBF5 antibody
      • CBF5 homolog antibody
      • Cbf5p homolog antibody
      • DKC 1 antibody
      • DKC antibody
      • Dkc1 antibody
      • DKC1_HUMAN antibody
      • DKCX antibody
      • Dyskeratosis congenita 1 antibody
      • Dyskeratosis congenita 1 dyskerin antibody
      • Dyskerin antibody
      • H/ACA ribonucleoprotein complex subunit 4 antibody
      • NAP 57 antibody
      • NAP57 antibody
      • NOLA 4 antibody
      • NOLA4 antibody
      • Nopp140 associated protein of 57 kDa antibody
      • Nopp140-associated protein of 57 kDa antibody
      • Nucleolar protein family A member 4 antibody
      • Nucleolar protein NAP57 antibody
      • snoRNP protein DKC1 antibody
      • XAP 101 antibody
      • XAP101 antibody
      see all

    Anti-DKC1 antibody images

    • ICC/IF image of ab93777 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab93777 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • All lanes : Anti-DKC1 antibody (ab93777) at 0.04 µg/ml

      Lane 1 : HeLa cell lysate at 50 µg
      Lane 2 : HeLa cell lysate at 15 µg
      Lane 3 : HeLa cell lysate at 5 µg
      Lane 4 : 293T cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 58 kDa


      Exposure time : 3 minutes
    • Detection of Human DKC1 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded)using ab93777 at 10 ug/mg lysate (LANE 1). DKC1 was also immunoprecipitated by an antibody which recognizes a downstream epitope (LANE 2). Lane 3 represents IgG Ip control. subsequent WB detection was performed using 1 ug/ml ab93777. Detection: Chemiluminescence with exposure times of 10 seconds.

    References for Anti-DKC1 antibody (ab93777)

    ab93777 has not yet been referenced specifically in any publications.

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