Overview

  • Product nameAnti-DKFZP434B168 antibody
    See all DKFZP434B168 primary antibodies
  • Description
    Rabbit polyclonal to DKFZP434B168
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human DKFZP434B168 aa 850-900.
    Database link: 25896

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 to 8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/10000 - 1/25000. Detects a band of approximately 110 kDa.

Target

  • FunctionComponent of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Plays a role in DNA damage response (DDR) signaling during the S phase.
  • Sequence similaritiesBelongs to the Integrator subunit 7 family.
  • Cellular localizationNucleus. Chromosome. Localizes to sites of DNA damage in a H2AX-independent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • INTS7 antibody
    • 5930412E23Rik antibody
    • C1orf73 antibody
    • Chromosome 1 open reading frame 73 antibody
    • chunp6916 antibody
    • DKFZp434B168 antibody
    • FLJ10736 antibody
    • FLJ12447 antibody
    • Int7 antibody
    • INT7_HUMAN antibody
    • Integrator complex subunit 7 antibody
    • Ints7 antibody
    • Ints7 integrator complex subunit 7 antibody
    • OTTHUMP00000035211 antibody
    • RGD1308014 antibody
    see all

Anti-DKFZP434B168 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling DKFZP434B168 with ab17256 at 1/1000 (1µg/ml). Detection: DAB.
  • Lane 1 : Anti-DKFZP434B168 antibody (ab17256) at 1/25000 dilution
    Lane 2 : Anti-DKFZP434B168 antibody (ab17256) at 1/10000 dilution

    Lane 1 : Nuclear lysates prepared from Hela cells
    Lane 2 : Nuclear lysates prepared from Hela cells


    Observed band size : 110 kDa (why is the actual band size different from the predicted?)

    Sample: Nuclear extract (NE) from HeLa cells.

    Antibody: ab17256 at 0.1 ug/ml. The band corresponding to DKFZP434B168 is arrowed at 110 kD. The identity of the other bands is unknown.

    Detection: Chemiluminescence with an exposure time of 15 minutes.

    We are unsure about the identity of other bands.

References for Anti-DKFZP434B168 antibody (ab17256)

ab17256 has not yet been referenced specifically in any publications.

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