The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: 1/100 - 1/500. Epitope exposure is recommended.
Epitope exposure with tris EDTA pH 9.0 buffer will enhance staining.
Likely to work with frozen sections
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionComponent of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Plays a role in DNA damage response (DDR) signaling during the S phase.
Sequence similaritiesBelongs to the Integrator subunit 7 family.
Cellular localizationNucleus. Chromosome. Localizes to sites of DNA damage in a H2AX-independent manner.