Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab109416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 29 kDa).
IHC-P 1/250 - 1/500. antigen retrieval is recommended.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionAntagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
    • Tissue specificityPlacenta.
    • Sequence similaritiesBelongs to the dickkopf family.
    • DomainThe C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Dickkopf 1 antibody
      • Dickkopf 1 homolog antibody
      • Dickkopf 1 like antibody
      • Dickkopf homolog 1 antibody
      • Dickkopf like protein 1 antibody
      • Dickkopf related protein 1 antibody
      • Dickkopf WNT signaling pathway inhibitor 1 antibody
      • Dickkopf-1 antibody
      • Dickkopf-related protein 1 antibody
      • DKK 1 antibody
      • Dkk-1 antibody
      • Dkk1 antibody
      • DKK1_HUMAN antibody
      • hDkk 1 antibody
      • hDkk-1 antibody
      • SK antibody
      see all

    Anti-DKK1 antibody [EPR4759] images

    • All lanes : Anti-DKK1 antibody [EPR4759] (ab109416) at 1/1000 dilution

      Lane 1 : JAR cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : NCCIT cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 29 kDa
      Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded Human placenta tissue using 1/100 ab109416
    • Immunofluorescent staining of HeLa cells using 1/100 ab109416

    References for Anti-DKK1 antibody [EPR4759] (ab109416)

    This product has been referenced in:
    • Li Y  et al. Minocycline protects against hepatic ischemia/reperfusion injury in a rat model. Biomed Rep 3:19-24 (2015). WB ; Rat . Read more (PubMed: 25469240) »
    • Chen C  et al. Elevated levels of Dickkopf-1 are associated with ß-catenin accumulation and poor prognosis in patients with chondrosarcoma. PLoS One 9:e105414 (2014). WB, IHC-P ; Human . Read more (PubMed: 25144498) »

    See all 4 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HUVEC)
    Loading amount 20 µg
    Specification HUVEC
    Gel Running Conditions Reduced Denaturing (8% gel)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Username

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    Verified customer

    Submitted Nov 16 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"