Overview

  • Product name
    Anti-DKK1 antibody [EPR4759]
    See all DKK1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4759] to DKK1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human DKK1 aa 250-350. The exact sequence is proprietary.

  • Positive control
    • JAR, HepG2, HeLa, and NCCIT cell lysates; Human placenta tissue; HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 29 kDa).
IHC-P 1/250 - 1/500. antigen retrieval is recommended.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
    • Tissue specificity
      Placenta.
    • Sequence similarities
      Belongs to the dickkopf family.
    • Domain
      The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Dickkopf 1 antibody
      • Dickkopf 1 homolog antibody
      • Dickkopf 1 like antibody
      • Dickkopf homolog 1 antibody
      • Dickkopf like protein 1 antibody
      • Dickkopf related protein 1 antibody
      • Dickkopf WNT signaling pathway inhibitor 1 antibody
      • Dickkopf-1 antibody
      • Dickkopf-related protein 1 antibody
      • DKK 1 antibody
      • Dkk-1 antibody
      • Dkk1 antibody
      • DKK1_HUMAN antibody
      • hDkk 1 antibody
      • hDkk-1 antibody
      • SK antibody
      see all

    Images

    • All lanes : Anti-DKK1 antibody [EPR4759] (ab109416) at 1/1000 dilution

      Lane 1 : JAR cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : NCCIT cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 29 kDa
      Observed band size: 38 kDa (why is the actual band size different from the predicted?)

    • Immunohistochemical analysis of paraffin-embedded Human placenta tissue using 1/100 ab109416
    • Immunofluorescent staining of HeLa cells using 1/100 ab109416

    References

    This product has been referenced in:
    • You S  et al. Tunicamycin inhibits colon carcinoma growth and aggressiveness via modulation of the ERK-JNK-mediated AKT/mTOR signaling pathway. Mol Med Rep 17:4203-4212 (2018). Read more (PubMed: 29344654) »
    • Kulkarni M  et al. Genome-wide analysis suggests a differential microRNA signature associated with normal and diabetic human corneal limbus. Sci Rep 7:3448 (2017). Read more (PubMed: 28615632) »

    See all 10 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HUVEC)
    Loading amount
    20 µg
    Specification
    HUVEC
    Gel Running Conditions
    Reduced Denaturing (8% gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 16 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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