The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 29 kDa).
1/250 - 1/500. antigen retrieval is recommended.
1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/100 - 1/250.
Application notesIs unsuitable for IP.
FunctionAntagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Sequence similaritiesBelongs to the dickkopf family.
DomainThe C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
Immunofluorescent staining of HeLa cells using 1/100 ab109416
References for Anti-DKK1 antibody [EPR4759] (ab109416)
This product has been referenced in:
Li Y et al. Minocycline protects against hepatic ischemia/reperfusion injury in a rat model. Biomed Rep3:19-24 (2015).
Read more (PubMed: 25469240) »
Chen C et al. Elevated levels of Dickkopf-1 are associated with ß-catenin accumulation and poor prognosis in patients with chondrosarcoma. PLoS One9:e105414 (2014).
Read more (PubMed: 25144498) »