DKK1 (Dickkopf-1) Human ELISA Kit (ab100501)

Overview

  • Product nameDKK1 (Dickkopf-1) Human ELISA KitSee all DKK1 kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 100 pg/ml
  • Range
    122.9 pg/ml - 30000 pg/ml
  • Recovery

    90 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 108.3 93% - 123%
    Serum 92.3 62% - 130%
    Plasma 72.8 59% - 110%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s DKK1 (Dickkopf-1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human DKK1 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human DKK1 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human DKK1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceDKK1 is an inhibitor of the Wnt signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
  • Alternative names
    • Dickkopf 1
    • Dickkopf homolog 1
    • Dickkopf related protein 1
    • DKK 1
    • hDkk 1
    • hDkk1
    • mDkk1
    • SK
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

DKK1 (Dickkopf-1) Human ELISA Kit images

  • Representative Standard Curve using ab100501.

  • Representative Standard Curve using ab100501.

Protocols

References for DKK1 (Dickkopf-1) Human ELISA Kit (ab100501)

ab100501 has not yet been referenced specifically in any publications.

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Thank you for your interest in our kits. I will send the product inserts first thing tomorrow morning, EST. The kits will be compatible with the automated ELISA machine, if the automated set-up approximates the conditions specified in the kit protocols...

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This kit has been shown that to cross-react with mouse samples, however, we do not have any information about cross-reactivity with rabbit samples.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"