Overview

  • Product name
  • Description
    Goat polyclonal to DKK2
  • Specificity
    May cross react with DKK4 due to sequence homology, not been tested.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Xenopus laevis
  • Immunogen

    Synthetic peptide:

    ATYSSKARLHVCQKI

    , corresponding to C terminal amino acids 245-259 of Human DKK2.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab21187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/8000.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 28 kDa.

Target

  • Function
    Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • Tissue specificity
    Expressed in heart, brain, skeletal muscle and lung.
  • Sequence similarities
    Belongs to the dickkopf family.
  • Domain
    The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
  • Post-translational
    modifications
    May be proteolytically processed by a furin-like protease.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dickkopf 2 antibody
    • Dickkopf gene 2 antibody
    • Dickkopf homolog 2 antibody
    • Dickkopf related protein 2 antibody
    • Dickkopf WNT signaling pathway inhibitor 2 antibody
    • Dickkopf-2 antibody
    • Dickkopf-related protein 2 antibody
    • Dickkopf2 antibody
    • DKK 2 antibody
    • Dkk-2 antibody
    • Dkk2 antibody
    • DKK2_HUMAN antibody
    • hDkk 2 antibody
    • hDkk-2 antibody
    • hDkk2 antibody
    see all

Images

  • ab21187 (2µg/ml) western blot detection of DKK2 from Human Heart lysate (35µg protein in RIPA buffer). Primary incubation of 1 hour, detected by chemiluminescence

References

This product has been referenced in:
  • Olivares-Navarrete R  et al. The roles of Wnt signaling modulators Dickkopf-1 (Dkk1) and Dickkopf-2 (Dkk2) and cell maturation state in osteogenesis on microstructured titanium surfaces. Biomaterials 31:2015-24 (2010). ELISA ; Human . Read more (PubMed: 20004015) »

See 1 Publication for this product

Customer reviews and Q&As

Abreviews
Application
ELISA
Sample
Mouse Recombinant protein (Recombinant mouse DKK-2 from R&D System)
Specification
Recombinant mouse DKK-2 from R&D System
Type
Sandwich (Capture)
Blocking step
FBS as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Ms. Jae Hun Shin

Verified customer

Submitted Jul 30 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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