The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 28 kDa.
FunctionAntagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Tissue specificityExpressed in heart, brain, skeletal muscle and lung.
Sequence similaritiesBelongs to the dickkopf family.
DomainThe C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
Post-translational modificationsMay be proteolytically processed by a furin-like protease.
ab21187 (2µg/ml) western blot detection of DKK2 from Human Heart lysate (35µg protein in RIPA buffer). Primary incubation of 1 hour, detected by chemiluminescence
References for Anti-DKK2 antibody (ab21187)
This product has been referenced in:
Olivares-Navarrete R et al. The roles of Wnt signaling modulators Dickkopf-1 (Dkk1) and Dickkopf-2 (Dkk2) and cell maturation state in osteogenesis on microstructured titanium surfaces. Biomaterials31:2015-24 (2010).
Read more (PubMed: 20004015) »