Overview

  • Product name
  • Description
    Rabbit polyclonal to DKK2
  • Specificity
    DKK2 fragments of ~55 kDa and ~28 kDa can also be detected in rat kidney tissue lysate
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, surrounding amino acid 241 of Mouse DKK2

  • Positive control
    • Lysate from Jurkat cells, 3T3 cells and rat kidney tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 30% Glycerol, 0.5% BSA, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    ab95274 is affinity purified.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 28 kDa.

Target

  • Function
    Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • Tissue specificity
    Expressed in heart, brain, skeletal muscle and lung.
  • Sequence similarities
    Belongs to the dickkopf family.
  • Domain
    The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
  • Post-translational
    modifications
    May be proteolytically processed by a furin-like protease.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dickkopf 2 antibody
    • Dickkopf gene 2 antibody
    • Dickkopf homolog 2 antibody
    • Dickkopf related protein 2 antibody
    • Dickkopf WNT signaling pathway inhibitor 2 antibody
    • Dickkopf-2 antibody
    • Dickkopf-related protein 2 antibody
    • Dickkopf2 antibody
    • DKK 2 antibody
    • Dkk-2 antibody
    • Dkk2 antibody
    • DKK2_HUMAN antibody
    • hDkk 2 antibody
    • hDkk-2 antibody
    • hDkk2 antibody
    see all

Images

  • Lane 1 : Anti-DKK2 antibody (ab95274) at 4 µg/ml
    Lanes 2 - 4 : Anti-DKK2 antibody (ab95274) at 4 µg/ml (in 1X TBS/T with 5% nonfat milk, overnight at 4C.)

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : 3T3 cell lysate
    Lane 4 : Rat kidney tissue lysate

    Secondary
    Anti-rabbit conjugated secondary antibody

    Predicted band size : 28 kDa

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab95274.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up