Overview

  • Product nameAnti-DKK2 antibody - Aminoterminal endSee all DKK2 primary antibodies ...
  • Description
    Rabbit polyclonal to DKK2 - Aminoterminal end
  • Tested applicationsELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a sequence within region aa 1-100 of human DKK2.

  • Positive control
    • Jurkat cell lysate; human hepatocarcinoma tissue.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab38594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IHC-P Use at an assay dependent dilution.

Target

  • FunctionAntagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • Tissue specificityExpressed in heart, brain, skeletal muscle and lung.
  • Sequence similaritiesBelongs to the dickkopf family.
  • DomainThe C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
  • Post-translational
    modifications
    May be proteolytically processed by a furin-like protease.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dickkopf 2 antibody
    • Dickkopf gene 2 antibody
    • Dickkopf homolog 2 antibody
    • Dickkopf related protein 2 antibody
    • Dickkopf WNT signaling pathway inhibitor 2 antibody
    • Dickkopf-2 antibody
    • Dickkopf-related protein 2 antibody
    • Dickkopf2 antibody
    • DKK 2 antibody
    • Dkk-2 antibody
    • Dkk2 antibody
    • DKK2_HUMAN antibody
    • hDkk 2 antibody
    • hDkk-2 antibody
    • hDkk2 antibody
    see all

Anti-DKK2 antibody - Aminoterminal end images

  • Anti-DKK2 antibody - Aminoterminal end (ab38594) at 1/100 dilution + Jurkat cell lysate

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
  • Formalin-fixed paraffin-embedded human hepatocarcinoma tissue reacted with ab38594, peroxidase-conjugated to the secondary antibody, followed by AEC staining.

References for Anti-DKK2 antibody - Aminoterminal end (ab38594)

This product has been referenced in:
  • Hauer K  et al. DKK2 mediates osteolysis, invasiveness, and metastatic spread in Ewing sarcoma. Cancer Res 73:967-77 (2013). Read more (PubMed: 23204234) »
  • Hassan MQ  et al. miR-218 Directs a Wnt Signaling Circuit to Promote Differentiation of Osteoblasts and Osteomimicry of Metastatic Cancer Cells. J Biol Chem 287:42084-92 (2012). WB ; Human . Read more (PubMed: 23060446) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Wound)
Specification Wound
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
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Submitted Sep 10 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (MDA-MB 436 breast cancer cells grown subcutaneousl)
Specification MDA-MB 436 breast cancer cells grown subcutaneousl
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate- EDTA (pH 6)
Permeabilization No
Blocking step Casein as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
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Submitted Oct 22 2009

Application Western blot
Sample Human Cell lysate - whole cell (MB-MDA-436 Breast Cancer Cells)
Loading amount 5 µg
Specification MB-MDA-436 Breast Cancer Cells
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
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Submitted Aug 05 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"