The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
Use at an assay dependent concentration.
FunctionAntagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Tissue specificityExpressed in heart, brain, skeletal muscle and lung.
Sequence similaritiesBelongs to the dickkopf family.
DomainThe C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
Post-translational modificationsMay be proteolytically processed by a furin-like protease.
Hassan MQ et al. miR-218 Directs a Wnt Signaling Circuit to Promote Differentiation of Osteoblasts and Osteomimicry of Metastatic Cancer Cells. J Biol Chem287:42084-92 (2012).
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