Overview

  • Product name
    Anti-DKK2 antibody - Aminoterminal end
    See all DKK2 primary antibodies
  • Description
    Rabbit polyclonal to DKK2 - Aminoterminal end
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a sequence within region aa 1-100 of human DKK2.

  • Positive control
    • Jurkat cell lysate; human hepatocarcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • Tissue specificity
    Expressed in heart, brain, skeletal muscle and lung.
  • Sequence similarities
    Belongs to the dickkopf family.
  • Domain
    The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
  • Post-translational
    modifications
    May be proteolytically processed by a furin-like protease.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dickkopf 2 antibody
    • Dickkopf gene 2 antibody
    • Dickkopf homolog 2 antibody
    • Dickkopf related protein 2 antibody
    • Dickkopf WNT signaling pathway inhibitor 2 antibody
    • Dickkopf-2 antibody
    • Dickkopf-related protein 2 antibody
    • Dickkopf2 antibody
    • DKK 2 antibody
    • Dkk-2 antibody
    • Dkk2 antibody
    • DKK2_HUMAN antibody
    • hDkk 2 antibody
    • hDkk-2 antibody
    • hDkk2 antibody
    see all

Images

  • Anti-DKK2 antibody - Aminoterminal end (ab38594) at 1/100 dilution + Jurkat cell lysate

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
  • Formalin-fixed paraffin-embedded human hepatocarcinoma tissue reacted with ab38594, peroxidase-conjugated to the secondary antibody, followed by AEC staining.

References

This product has been referenced in:
  • Mu J  et al. Dickkopf-related protein 2 induces G0/G1 arrest and apoptosis through suppressing Wnt/ß-catenin signaling and is frequently methylated in breast cancer. Oncotarget 8:39443-39459 (2017). WB, IF, IHC . Read more (PubMed: 28467796) »
  • Hauer K  et al. DKK2 mediates osteolysis, invasiveness, and metastatic spread in Ewing sarcoma. Cancer Res 73:967-77 (2013). Read more (PubMed: 23204234) »

See all 5 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Wound)
Specification
Wound
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Sep 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (MDA-MB 436 breast cancer cells grown subcutaneousl)
Specification
MDA-MB 436 breast cancer cells grown subcutaneousl
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate- EDTA (pH 6)
Permeabilization
No
Blocking step
Casein as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 22 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MB-MDA-436 Breast Cancer Cells)
Loading amount
5 µg
Specification
MB-MDA-436 Breast Cancer Cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Aug 05 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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