Anti-Dkk3 antibody (ab214360)
Key features and details
- Rabbit polyclonal to Dkk3
- Suitable for: IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Dkk3 antibody
See all Dkk3 primary antibodies -
Description
Rabbit polyclonal to Dkk3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Human Dkk3 aa 50-150 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9UBP4 -
Positive control
- Human lung carcinoma and rat buccal mucosa cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab214360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500.
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Notes |
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IHC-P
1/100 - 1/500. |
Target
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Function
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. -
Tissue specificity
Highest expression in heart, brain, and spinal cord. -
Sequence similarities
Belongs to the dickkopf family. -
Domain
The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 27122 Human
- Entrez Gene: 50781 Mouse
- Entrez Gene: 171548 Rat
- Omim: 605416 Human
- SwissProt: Q9UBP4 Human
- SwissProt: Q9QUN9 Mouse
- Unigene: 292156 Human
- Unigene: 55143 Mouse
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Alternative names
- Dickkopf 3 antibody
- Dickkopf 3 homolog (Xenopus laevis) antibody
- Dickkopf 3 homolog antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma labeling Dkk3 with ab214360 at 1/100 dilution followed by conjugation to the secondary antibody and DAB staining.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat buccal mucosa cancer labeling Dkk3 with ab214360 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab214360 has been referenced in 1 publication.
- Liu Y et al. RIG-I Promotes Cell Viability, Colony Formation, and Glucose Metabolism and Inhibits Cell Apoptosis in Colorectal Cancer by NF-κB Signaling Pathway. Dis Markers 2022:1247007 (2022). PubMed: 35242239