Overview

  • Product name
  • Description
    Goat polyclonal to Dkk3
  • Specificity
    This antibody is expected to recognise the 350 aa isoform only.
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    AAAALLGGEEI

    , corresponding to C terminal amino acids 340-350 of 350 aa isoform DKK3.

  • Positive control
    • 293 lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.03 - 0.1 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 25029272
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • Tissue specificity
    Highest expression in heart, brain, and spinal cord.
  • Sequence similarities
    Belongs to the dickkopf family.
  • Domain
    The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dickkopf 3 antibody
    • Dickkopf 3 homolog (Xenopus laevis) antibody
    • Dickkopf 3 homolog antibody
    • Dickkopf homolog 3 antibody
    • Dickkopf related protein 3 antibody
    • Dickkopf related protein 3 Precursor antibody
    • Dickkopf WNT signaling pathway inhibitor 3 antibody
    • Dickkopf, Xenopus, homolog of, 3 antibody
    • Dickkopf-3 antibody
    • Dickkopf-related protein 3 antibody
    • Dickkopf3 antibody
    • DKK 3 antibody
    • Dkk-3 antibody
    • DKK3 antibody
    • DKK3_HUMAN antibody
    • hDkk 3 antibody
    • hDkk-3 antibody
    • hDkk3 antibody
    • Regulated in glioma antibody
    • REIC antibody
    • RIG antibody
    • RIG-like 5-6 antibody
    • RIG-like 7-1 antibody
    see all

Images

  • Ab2459 staining (2µg/ml) of 293 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.
  • ab2459 staining DKK3 in human MDA-MB 436 cells by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were paraformaldehyde fixed prior to blocking in 10% casein for 30 minutes at 22°C. The primary antibody was diluted 1/75 and incubated with the sample for 30 minutes at 22°C. A biotin conjugated rabbit polyclonal to goat Ig, diluted 1/200, was used as the secondary.

    See Abreview

References

This product has been referenced in:
  • Marthandan S  et al. Conserved Senescence Associated Genes and Pathways in Primary Human Fibroblasts Detected by RNA-Seq. PLoS One 11:e0154531 (2016). WB . Read more (PubMed: 27140416) »
  • Marthandan S  et al. Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts. Biomed Res Int 2015:731938 (2015). WB . Read more (PubMed: 26339636) »

See all 4 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human MDA-MB-436 Breast Cancer Cells)
Loading amount
3 µg
Specification
Human MDA-MB-436 Breast Cancer Cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
1xCasein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Apr 27 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human MDA-MB 436 cells grown subcutaneously in mou)
Specification
Human MDA-MB 436 cells grown subcutaneously in mou
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate-EDTA (pH6)
Permeabilization
No
Blocking step
casein as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 27 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - other (Cerebrospinal fluid (CSF))
Loading amount
3 µg
Specification
Cerebrospinal fluid (CSF)
Gel Running Conditions
Reduced Denaturing (12% SDS)
Blocking step
Milk as blocking agent for 17 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: + 4°C
Username

Ms. Christiane Rewerts

Verified customer

Submitted Jun 04 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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