Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    Western Blot – Approx 30-35kDa bands seen in Jurkat lysate.
    Recommended for use at 1-3µg/ml.

    Please note that literature indicates DKK4 is detected as three major immunoreactive species of approx. 40 kDa [form (i)], 30-32 kDa [form (ii)] and 15-17 kDa [form (iii)]. We believe this antibody will recognise 30-32kDa species of DKK4.
  • Target

    • Function
      Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
    • Tissue specificity
      Expressed in cerebellum, T-cells, esophagus and lung.
    • Sequence similarities
      Belongs to the dickkopf family.
    • Domain
      The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
    • Post-translational
      modifications
      Appears to be not glycosylated.
      Can be proteolytically processed by a furin-like protease.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Dickkopf 4 antibody
      • Dickkopf gene 4 antibody
      • Dickkopf homolog 4 (Xenopus laevis) antibody
      • Dickkopf homolog 4 antibody
      • Dickkopf related protein 4 antibody
      • Dickkopf-4 antibody
      • Dickkopf-related protein 4 short form antibody
      • Dickkopf4 antibody
      • DKK 4 antibody
      • Dkk-4 antibody
      • DKK4 antibody
      • DKK4_HUMAN antibody
      • hDkk 4 antibody
      • hDkk-4 antibody
      • hDkk4 antibody
      • MGC129562 antibody
      • MGC129563 antibody
      see all

    Images

    • Ab2342 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence.

    References

    ab2342 has not yet been referenced specifically in any publications.

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    There are currently no Customer reviews or Questions for ab2342.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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