The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
Use at an assay dependent concentration.
FunctionAntagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Tissue specificityExpressed in cerebellum, T-cells, esophagus and lung.
Sequence similaritiesBelongs to the dickkopf family.
DomainThe C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
Post-translational modificationsAppears to be not glycosylated. Can be proteolytically processed by a furin-like protease.