Overview

  • Product name
  • Description
    Rabbit polyclonal to DKK4
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (10-30 aa in length) conjugated to KLH and derived from from the last C terminal 50 amino acids of DKK4 (Human)

  • Positive control
    • A375 cell lysate; Human hepatocarcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody was eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • Tissue specificity
    Expressed in cerebellum, T-cells, esophagus and lung.
  • Sequence similarities
    Belongs to the dickkopf family.
  • Domain
    The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
  • Post-translational
    modifications
    Appears to be not glycosylated.
    Can be proteolytically processed by a furin-like protease.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dickkopf 4 antibody
    • Dickkopf gene 4 antibody
    • Dickkopf homolog 4 (Xenopus laevis) antibody
    • Dickkopf homolog 4 antibody
    • Dickkopf related protein 4 antibody
    • Dickkopf-4 antibody
    • Dickkopf-related protein 4 short form antibody
    • Dickkopf4 antibody
    • DKK 4 antibody
    • Dkk-4 antibody
    • DKK4 antibody
    • DKK4_HUMAN antibody
    • hDkk 4 antibody
    • hDkk-4 antibody
    • hDkk4 antibody
    • MGC129562 antibody
    • MGC129563 antibody
    see all

Images

  • Anti-DKK4 antibody (ab38589) + A375 cell lysate

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
    Additional bands at : 30 kDa,35 kDa. We are unsure as to the identity of these extra bands.
  • Formalin-fixed and paraffin-embedded human cancer tissue (hepatocarcinoma) with ab38589 DKK4 antibody, peroxidase-conjugated to the secondary antibody, followed by AEC staining.

References

This product has been referenced in:
  • Pfaff EM  et al. Dickkopf proteins influence lung epithelial cell proliferation in idiopathic pulmonary fibrosis. Eur Respir J : (2010). Read more (PubMed: 20650998) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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