The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
FunctionMay act as a tumor suppressor by inhibiting cell proliferation.
Tissue specificityExpressed in all tissues examined. Expression is highest in prostate and testis.
Involvement in diseaseDLEC1 silencing due to promoter methylation and aberrant transcription are implicated in the development of different cancers, including esophageal (ESCR), renal and lung cancers (LNCR). Lung cancer Esophageal cancer