The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 21294127
Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
FunctionMay play a role at the plasma membrane in the maintenance of the structure of epithelial cells and in the transmission of extracellular signals to the membrane and cytoskeleton.
Tissue specificityHighly expressed in the placenta and prostate. Expressed at a lower level in the thyroid, spinal cord, trachea, adrenal gland, skeletal muscle, pancreas, heart, brain, liver and kidney. A short splice product shows more limited expression, being absent from at least the brain.
Sequence similaritiesBelongs to the MAGUK family. Contains 1 CARD domain. Contains 1 guanylate kinase-like domain. Contains 4 PDZ (DHR) domains. Contains 1 SH3 domain.
Cellular localizationCell junction. Cell membrane. Localized at sites of cell-cell contact.
Ruiz C et al. Amplification and overexpression of vinculin are associated with increased tumour cell proliferation and progression in advanced prostate cancer. J Pathol223:543-52 (2011).
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