Overview

  • Product name
    Anti-DLG5 antibody
  • Description
    Rabbit polyclonal to DLG5
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1325 and 1375 of human DLG5 (NP_004738.3).

  • Positive control
    • HeLa whole cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate buffer, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab86783 was affinity purified using an epitope specific to DLG5 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 10 µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 214 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      May play a role at the plasma membrane in the maintenance of the structure of epithelial cells and in the transmission of extracellular signals to the membrane and cytoskeleton.
    • Tissue specificity
      Highly expressed in the placenta and prostate. Expressed at a lower level in the thyroid, spinal cord, trachea, adrenal gland, skeletal muscle, pancreas, heart, brain, liver and kidney. A short splice product shows more limited expression, being absent from at least the brain.
    • Sequence similarities
      Belongs to the MAGUK family.
      Contains 1 CARD domain.
      Contains 1 guanylate kinase-like domain.
      Contains 4 PDZ (DHR) domains.
      Contains 1 SH3 domain.
    • Cellular localization
      Cell junction. Cell membrane. Localized at sites of cell-cell contact.
    • Information by UniProt
    • Database links
    • Alternative names
      • Discs large homolog 5 (Drosophila) antibody
      • Discs large homolog 5 antibody
      • Discs large protein LP DLG antibody
      • Discs large protein P dlg antibody
      • Discs large protein P-dlg antibody
      • Disks large homolog 5 antibody
      • DLG 5 antibody
      • DLG5 antibody
      • DLG5_HUMAN antibody
      • KIAA0583 antibody
      • Large type of P DLG antibody
      • LP DLG antibody
      • P DLG5 antibody
      • PDLG antibody
      • Placenta and prostate DLG antibody
      see all

    Images

    • All lanes : Anti-DLG5 antibody (ab86783) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 214 kDa


      Exposure time : 3 minutes
    • Detection of DLG5 by Western Blot of Immunprecipitate.
      ab86783, at 1 µg/ml, staining DLG5 in HeLa whole cell lysate immunoprecipitated using ab86783 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with an exposure time of 30 seconds.

    References

    ab86783 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab86783.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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