Anti-DLK-1 antibody [3A10] (ab119930)
Key features and details
- Mouse monoclonal [3A10] to DLK-1
- Suitable for: WB, ELISA, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-DLK-1 antibody [3A10]
See all DLK-1 primary antibodies -
Description
Mouse monoclonal [3A10] to DLK-1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISA, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human, Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human DLK-1 aa 174-349.
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Positive control
- Recombinant DLK-1 protein; U251 cells; NIH3T3 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
3A10 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119930 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 41 kDa.
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ELISA |
1/10000.
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Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
ICC/IF | (2) |
1/200 - 1/1000.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 41 kDa. |
ELISA
1/10000. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/200 - 1/1000. |
Target
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Function
May have a role in neuroendocrine differentiation. -
Tissue specificity
Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue. -
Sequence similarities
Contains 6 EGF-like domains. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 8788 Human
- Entrez Gene: 13386 Mouse
- Omim: 176290 Human
- SwissProt: P80370 Human
- SwissProt: Q09163 Mouse
- Unigene: 533717 Human
- Unigene: 157069 Mouse
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Alternative names
- Adipocyte differentiation inhibitor protein antibody
- Brevideltin antibody
- Delta like 1 homolog antibody
see all
Images
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Immunofluorescence analysis of U251 cells using ab119930 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Anti-DLK-1 antibody [3A10] (ab119930) at 1/500 dilution + Recombinant DLK-1 protein
Predicted band size: 41 kDa -
Flow cytometric analysis of NIH3T3 cells using ab119930 at 1/200 dilution (green) and negative control (red).
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ELISA using ab119930.
Protocols
Datasheets and documents
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Datasheet download
References (11)
ab119930 has been referenced in 11 publications.
- Bondareva O et al. Single-cell profiling of vascular endothelial cells reveals progressive organ-specific vulnerabilities during obesity. Nat Metab 4:1591-1610 (2022). PubMed: 36400935
- Eze UC et al. Single-cell atlas of early human brain development highlights heterogeneity of human neuroepithelial cells and early radial glia. Nat Neurosci 24:584-594 (2021). PubMed: 33723434
- Shen AH et al. Prophylactic treatment with transdermal deferoxamine mitigates radiation-induced skin fibrosis. Sci Rep 10:12346 (2020). PubMed: 32704071
- Wise LM et al. Anti-fibrotic Actions of Equine Interleukin-10 on Transforming Growth Factor-Beta1-Stimulated Dermal Fibroblasts Isolated From Limbs of Horses. Front Vet Sci 7:577835 (2020). PubMed: 33195583
- Borrelli MR et al. Fat grafting rescues radiation-induced joint contracture. Stem Cells 38:382-389 (2020). PubMed: 31793745