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Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human DLK.
(Peptide available as ab23442.)
Our Abpromise guarantee covers the use of ab21682 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/150. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
|WB||1/2000. Detects a band of approximately 45,48 kDa (predicted molecular weight: 41 kDa).
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product.
Immohistochemical analysis of formaldehyde-fixed paraffin-embedded human placental tissue sections, labelling DLK with ab21682 at a dilution of 1/1000 incubated for 20 minutes at 25°C. Blocking was with hydrogen peroxide at 3% incubated for 10 minutes at 25°C. The secondary used was a gekko ant-rabbit polyclonal HRP conjugate used undiluted.
This blot was produced using an 8% Tris-glycine gel under the Tris-glycine buffer system. The gel was run at 120V for 1 hour 15 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab21682 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Image courtesy of Human Protein Atlas
ab21682 staining DLK in human bone marrow within the haemopoietic tissue and not the adipocytes. Paraffin embedded human bone marrow was incubated with ab21682 (1/150 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab21682 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org.