Overview

  • Product nameAnti-DLK antibody
    See all DLK primary antibodies
  • Description
    Rabbit polyclonal to DLK
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human DLK.

    (Peptide available as ab23442.)

  • Positive control
    • This antibody gave a positive signal in E18.5 Mouse Muscle Wild Type Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab21682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/150. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB 1/2000. Detects a band of approximately 45,48 kDa (predicted molecular weight: 41 kDa).

Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product.

Target

  • FunctionMay have a role in neuroendocrine differentiation.
  • Tissue specificityFound within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue.
  • Sequence similaritiesContains 6 EGF-like domains.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adipocyte differentiation inhibitor protein antibody
    • Brevideltin antibody
    • Delta like 1 homolog antibody
    • Delta like homolog antibody
    • Delta like protein antibody
    • Delta1 antibody
    • DLK 1 antibody
    • DLK antibody
    • DLK-1 antibody
    • DLK1 antibody
    • DLK1_HUMAN antibody
    • FA 1 antibody
    • FA1 antibody
    • Fetal antigen 1 antibody
    • pG2 antibody
    • Preadipocyte factor 1 antibody
    • Pref 1 antibody
    • Pref antibody
    • Pref1 antibody
    • Protein delta homolog 1 antibody
    • Secredeltin antibody
    • ZOG antibody
    see all

Anti-DLK antibody images

  • Immohistochemical analysis of formaldehyde-fixed paraffin-embedded human placental tissue sections, labelling DLK with ab21682 at a dilution of 1/1000 incubated for 20 minutes at 25°C. Blocking was with hydrogen peroxide at 3% incubated for 10 minutes at 25°C. The secondary used was a gekko ant-rabbit polyclonal HRP conjugate used undiluted.

    See Abreview

  • All lanes : Anti-DLK antibody (ab21682) at 1/2000 dilution ((5% Milk blocking))

    Lane 1 : E18.5 Mouse Muscle Wild Type Tissue Lysate
    Lane 2 : E18.5 Mouse Muscle DLK1 Knockout Tissue Lysate

    Lysates/proteins at 5 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 45,48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 15 kDa (possible cleavage fragment),55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

    This blot was produced using an 8% Tris-glycine gel under the Tris-glycine buffer system. The gel was run at 120V for 1 hour 15 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab21682 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

     

    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

  • Image courtesy of Human Protein Atlas

    ab21682 staining DLK in human bone marrow within the haemopoietic tissue and not the adipocytes. Paraffin embedded human bone marrow was incubated with ab21682 (1/150 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab21682 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org.

References for Anti-DLK antibody (ab21682)

This product has been referenced in:
  • Kim KH  et al. CCN1 induces hepatic ductular reaction through integrin avß5-mediated activation of NF-?B. J Clin Invest 125:1886-900 (2015). ICC/IF ; Mouse . Read more (PubMed: 25822023) »
  • Charalambous M  et al. DLK1/PREF1 regulates nutrient metabolism and protects from steatosis. Proc Natl Acad Sci U S A 111:16088-93 (2014). Read more (PubMed: 25349437) »

See all 11 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (placenta)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate, pH6, 100C, 20 min
Permeabilization No
Specification placenta
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 19 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Mouse Cell (neurons)
Specification neurons
Permeabilization Yes - 0.2% Tween 20
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 22 2014

Muchas gracias por tu paciencia.

Lamento la espera. Mis compañeros del laboratorio me han confirmado que este anticuerpo solo lo han testado en células MES en inmunocitoquímica, y en WB en lisados de MEL-1, F9 y Mouse EG....

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x
Je suis ravie que vous souhaitiez participer a notre offre et tester ab21682 en cytométrie de flux. Le code de réduction correspondant est xx . Ce code de réduction est utilisable sous 120 jours et pour l’achat d’un autre anticorps primaire.
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Thank you for these precisions.
We are still working on your complaint for ab21682 (anti-DLK1) and we will come back to you as soon as possible.
As requested, I have issued a free of charge replacement with the order number *******.
To che...

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Thank you very much for your email and your patience.
In regards to the first issue, with the purification of ab110363, I had contacted the laboratory. There is nothing special with this clone. The only thing which might could explain the result i...

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Thank you for calling Abcam last night.

I have spoken to my colleagues about your wish to return the 2 vials of ab21682. Since they were purchased more than 2 weeks ago, we cannot accept them as a return, I am sorry about that. I understand t...

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Thank you for contacting us regarding this possibility. I have contacted the lab regarding the possibility that this product is recognizing the extracellular domain. I’m afraid we have no testing data to show the specificity for the intracellu...

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Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Embryonic stem cell-derived hepatocyte precursors)
Specification Embryonic stem cell-derived hepatocyte precursors
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Irina Drobinskaya

Verified customer

Submitted Feb 12 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"