• Product nameAnti-DLK antibody [MM0514-9D8]
    See all DLK primary antibodies
  • Description
    Mouse monoclonal [MM0514-9D8] to DLK
  • Tested applicationsSuitable for: IHC-P, WB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Human DLK protein.



Our Abpromise guarantee covers the use of ab89908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 41 kDa.
Flow Cyt Use 2µg for 106 cells. We recommend to fix with 4% formaldehyde for 10min at room temperature.

ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml.


  • FunctionMay have a role in neuroendocrine differentiation.
  • Tissue specificityFound within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue.
  • Sequence similaritiesContains 6 EGF-like domains.
  • Post-translational
    N- and O-glycosylated.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adipocyte differentiation inhibitor protein antibody
    • Brevideltin antibody
    • Delta like 1 homolog antibody
    • Delta like homolog antibody
    • Delta like protein antibody
    • Delta1 antibody
    • DLK 1 antibody
    • DLK antibody
    • DLK-1 antibody
    • DLK1 antibody
    • DLK1_HUMAN antibody
    • FA 1 antibody
    • FA1 antibody
    • Fetal antigen 1 antibody
    • pG2 antibody
    • Preadipocyte factor 1 antibody
    • Pref 1 antibody
    • Pref antibody
    • Pref1 antibody
    • Protein delta homolog 1 antibody
    • Secredeltin antibody
    • ZOG antibody
    see all

Anti-DLK antibody [MM0514-9D8] images

  • ICC/IF image of ab89908 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89908, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HepG2 cells stained with ab89908 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89908, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min) used under the same conditions.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • IHC image of ab89908 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab89908, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-DLK antibody [MM0514-9D8] (ab89908)

This product has been referenced in:
  • Guye P  et al. Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6. Nat Commun 7:10243 (2016). IF ; Human . Read more (PubMed: 26732624) »

See 1 Publication for this product

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Thank you for contacting us. This antibody was validated in Flow Cytometry using a human glioma cell line (A172). I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us and your interest in our products. I am pleased to provide the following immunogen information: The immunogen for this clone is a recombinant peptide between aa 150 to aa297 of human DLK protein (P80370). This correspo...

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