Overview

  • Product name
    Anti-DLK2 antibody - C-terminal
    See all DLK2 primary antibodies
  • Description
    Rabbit polyclonal to DLK2 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human DLK2 aa 354-383 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q6UY11

  • Positive control
    • HL60 cell lysate; Human brain tissue; HL60 cells; 293 cell lysate transiently transfected with the DLK2 gene.

Properties

Applications

Our Abpromise guarantee covers the use of ab176003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 40 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Regulates adipogenesis.
  • Sequence similarities
    Contains 6 EGF-like domains.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI413481 antibody
    • Delta like 2 homolog antibody
    • Delta like protein 2 antibody
    • delta-like 2 homolog (Drosophila) antibody
    • DLK 2 antibody
    • DLK-2 antibody
    • Dlk2 antibody
    • DLK2_HUMAN antibody
    • EGF like domain containing protein 9 antibody
    • EGF like domain multiple 9 antibody
    • EGF-like protein 9 antibody
    • EGFL 9 antibody
    • EGFL9 antibody
    • Epidermal growth factor-like protein 9 antibody
    • MGC111055 antibody
    • MGC2487 antibody
    • Multiple EGF like domain protein 9 antibody
    • OTTHUMP00000016449 antibody
    • OTTHUMP00000016451 antibody
    • Protein delta homolog 2 antibody
    see all

Images

  • All lanes : Anti-DLK2 antibody - C-terminal (ab176003) at 1/1000 dilution

    Lane 1 : Non-transfected 293 cell lysate
    Lane 2 : 293 cell lysate transiently transfected with the DLK2 gene

    Lysates/proteins at 2 µg per lane.

    Predicted band size: 40 kDa

  • Flow cytometric analysis of HL60 cells labeling DLK2 with ab176003 at 1/10 dilution (green) compared to negative control cells (blue). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling DLK2 with ab176003 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-DLK2 antibody - C-terminal (ab176003) at 1/1000 dilution + HL60 cell lysate at 35 µg

    Predicted band size: 40 kDa

References

ab176003 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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