• Product nameAnti-DLL1 antibody
    See all DLL1 primary antibodies
  • Description
    Rabbit polyclonal to DLL1
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Fr, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Chicken, Human
  • Immunogen

    Synthetic peptide:


    conjugated to KLH, corresponding to amino acids 155-173 of Mouse Delta-1.

  • General notesDilute only prior to immediate use.



Our Abpromise guarantee covers the use of ab10554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 78 kDa.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.


  • FunctionActs as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
  • Tissue specificityExpressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.
  • Sequence similaritiesContains 1 DSL domain.
    Contains 8 EGF-like domains.
  • Post-translational
    Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Delta (Drosophila) like 1 antibody
    • Delta antibody
    • Delta like 1 (Drosophila) antibody
    • Delta like 1 antibody
    • Delta like 1 protein antibody
    • Delta like protein 1 antibody
    • Delta like protein 1 precursor antibody
    • Delta, Drosophila, homolog of 1 antibody
    • Delta-like protein 1 antibody
    • Delta1 antibody
    • DL1 antibody
    • DLL 1 antibody
    • DLL1 antibody
    • DLL1_HUMAN antibody
    • Drosophila Delta homolog 1 antibody
    • H-Delta-1 antibody
    • OTTHUMP00000017690 antibody
    see all

Anti-DLL1 antibody images

  • Predicted band size : 78 kDa

    Western blot using Delta 1 antibody (ab10554) at 1/500 tested on the following tissue lysates:

    Lane 1: Mouse Heart
    Lane 2: Mouse Pancreas
    Lane 3: Mouse Brain
    Lane 4: Rat Heart
    Lane 5: Rat Pancreas
    Lane 6: Rat Brain

    Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
    Exposure time: 1 minute
    Expected molecular weight: 77kDa
    Cell lysates at 20µg per lane.

    NB: This image shows that is was not possible to detect Delta 1 immunoreactivity by WB in the lysates tested. However little is known about Delta 1 expression and it is likely to be developmentally regulated with low expression/abundance making it difficult to detect in whole tissue homogenates. In favour of Delta 1 antibody (ab10554), the staining on the WB shows very low background and little cross reactivity in whole homogenates.

  • ICC/IF image of ab10554 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10554, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-DLL1 antibody (ab10554)

This product has been referenced in:
  • Murta D  et al. In vivo notch signaling blockade induces abnormal spermatogenesis in the mouse. PLoS One 9:e113365 (2014). IHC ; Mouse . Read more (PubMed: 25412258) »
  • Arboleda-Velasquez JF  et al. Notch signaling functions in retinal pericyte survival. Invest Ophthalmol Vis Sci 55:5191-9 (2014). WB . Read more (PubMed: 25015359) »

See all 6 Publications for this product

Product Wall

Thank you for your email. Ab10554 is a Fast-Track antibody - Fast-Track antibodies are immunogen affinity purified and are shown by ELISA to have high titre values against their immunogen. We are currently testing them for use in Western blotting. At t...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cow Tissue sections (Liver)
Specification Liver
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization Yes - Triton
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C

Dr. Daniel Murta

Verified customer

Submitted May 15 2013

Unfortunately it is not possible for us to sell the pre-immune serum of our rabbits.
I am sorry that on this occasion I could not be of more help.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Intestine)
Specification Intestine
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton 20 min at RT
Blocking step Protein Block as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Adam Gracz

Verified customer

Submitted Jan 04 2013

This Rabbit polyclonal antibody to Delta 1 has been shown to work in ELISA against the peptide immunogen but has not been shown to detect the endogenous protein (WB) in the lysates specified on the datsheet. The anitbody is sold as a special investigat...

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Thank you for your enquiry. We cannot give you an exact time before which the signal will fade with these fluorophore conjugated secondary antibodies. The signal could last a week (the time frame that you mentioned that you would need) if your samples ...

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