• Product name
  • Description
    Rabbit polyclonal to DLL1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (GSRCALALAV LSALLCQVWS SGVFELKLQE FVNKKGLLGN RNCCRGGAGP) of Human DLL1 (NP_005609).

  • Positive control
    • Hela cell lysate



Our Abpromise guarantee covers the use of ab84620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 78 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • Function
    Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
  • Tissue specificity
    Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.
  • Sequence similarities
    Contains 1 DSL domain.
    Contains 8 EGF-like domains.
  • Post-translational
    Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Delta (Drosophila) like 1 antibody
    • Delta antibody
    • Delta like 1 (Drosophila) antibody
    • Delta like 1 antibody
    • Delta like 1 protein antibody
    • Delta like protein 1 antibody
    • Delta like protein 1 precursor antibody
    • Delta, Drosophila, homolog of 1 antibody
    • Delta-like protein 1 antibody
    • Delta1 antibody
    • DL1 antibody
    • DLL 1 antibody
    • DLL1 antibody
    • DLL1_HUMAN antibody
    • Drosophila Delta homolog 1 antibody
    • H-Delta-1 antibody
    • OTTHUMP00000017690 antibody
    see all


  • Anti-DLL1 antibody (ab84620) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 78 kDa
    Observed band size : 78 kDa
  • IHC image of ab84620 staining in heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84620, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:
  • Robinson SC  et al. Kaiso differentially regulates components of the Notch signaling pathway in intestinal cells. Cell Commun Signal 15:24 (2017). Read more (PubMed: 28637464) »
  • Singh NK  et al. Cyclic AMP Response Element Binding Protein Mediates Pathological Retinal Neovascularization via Modulating DLL4-NOTCH1 Signaling. EBioMedicine 2:1767-84 (2015). Read more (PubMed: 26870802) »

See all 6 Publications for this product

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