Anti-DLL1 antibody (ab85346)
Key features and details
- Goat polyclonal to DLL1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-DLL1 antibody
See all DLL1 primary antibodies -
Description
Goat polyclonal to DLL1 -
Host species
Goat -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Xenopus laevis, Zebrafish -
Immunogen
Synthetic peptide:
C-ATQRHLTVGEEWSQD
, corresponding to internal sequence amino acids 148-162 of Human DLL1 (NP_005609.3) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab85346 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | (1) |
Use at an assay dependent concentration.
|
WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. -
Tissue specificity
Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney. -
Sequence similarities
Contains 1 DSL domain.
Contains 8 EGF-like domains. -
Post-translational
modificationsUbiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 28514 Human
- Entrez Gene: 13388 Mouse
- Entrez Gene: 84010 Rat
- Omim: 606582 Human
- SwissProt: O00548 Human
- SwissProt: Q61483 Mouse
- SwissProt: P97677 Rat
- Unigene: 379912 Human
see all -
Alternative names
- Delta (Drosophila) like 1 antibody
- Delta antibody
- Delta like 1 (Drosophila) antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab85346 has been referenced in 9 publications.
- Gao L et al. Receptor-selective interleukin-4 mutein attenuates laser-induced choroidal neovascularization through the regulation of macrophage polarization in mice. Exp Ther Med 22:1367 (2021). PubMed: 34659513
- Banerjee A et al. Dengue virus infection impedes megakaryopoiesis in MEG-01 cells where the virus envelope protein interacts with the transcription factor TAL-1. Sci Rep 10:19587 (2020). PubMed: 33177556
- Song Y et al. The ethyl acetate extracts of radix trichosanthis protect retinal vascular endothelial cells from high glucose-induced injury. J Ethnopharmacol 240:111954 (2019). PubMed: 31085225
- Tetzlaff F et al. MPDZ promotes DLL4-induced Notch signaling during angiogenesis. Elife 7:N/A (2018). PubMed: 29620522
- Wang Z et al. Folic Acid Has a Protective Effect on Retinal Vascular Endothelial Cells against High Glucose. Molecules 23:N/A (2018). PubMed: 30213067
- Scavuzzo MA et al. Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA. Cell Rep 25:3811-3827.e7 (2018). PubMed: 30590051
- Chen KY et al. A Notch positive feedback in the intestinal stem cell niche is essential for stem cell self-renewal. Mol Syst Biol 13:927 (2017). WB . PubMed: 28455349
- Braune EB et al. S/T phosphorylation of DLL1 is required for full ligand activity in vitro but dispensable for DLL1 function in vivo during embryonic patterning and marginal zone B cell development. Mol Cell Biol 34:1221-33 (2014). PubMed: 24449764
- El-Kassar N et al. High levels of IL-7 cause dysregulation of thymocyte development. Int Immunol 24:661-71 (2012). PubMed: 22899673