Overview

  • Product nameAnti-DLL1 antibody
    See all DLL1 primary antibodies
  • Description
    Goat polyclonal to DLL1
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide:

    C-ATQRHLTVGEEWSQD

    , corresponding to internal sequence amino acids 148-162 of Human DLL1 (NP_005609.3)

  • Positive control
    • Mouse Heart and Rat Heart lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab85346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).

Target

  • FunctionActs as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
  • Tissue specificityExpressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.
  • Sequence similaritiesContains 1 DSL domain.
    Contains 8 EGF-like domains.
  • Post-translational
    modifications
    Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Delta (Drosophila) like 1 antibody
    • Delta antibody
    • Delta like 1 (Drosophila) antibody
    • Delta like 1 antibody
    • Delta like 1 protein antibody
    • Delta like protein 1 antibody
    • Delta like protein 1 precursor antibody
    • Delta, Drosophila, homolog of 1 antibody
    • Delta-like protein 1 antibody
    • Delta1 antibody
    • DL1 antibody
    • DLL 1 antibody
    • DLL1 antibody
    • DLL1_HUMAN antibody
    • Drosophila Delta homolog 1 antibody
    • H-Delta-1 antibody
    • OTTHUMP00000017690 antibody
    see all

Anti-DLL1 antibody images

  • Anti-DLL1 antibody (ab85346) at 0.3 µg/ml + Rat Heart lysate at 35 µg

    Predicted band size : 78 kDa
    Observed band size : 78 kDa

References for Anti-DLL1 antibody (ab85346)

This product has been referenced in:
  • Braune EB  et al. S/T phosphorylation of DLL1 is required for full ligand activity in vitro but dispensable for DLL1 function in vivo during embryonic patterning and marginal zone B cell development. Mol Cell Biol 34:1221-33 (2014). Read more (PubMed: 24449764) »
  • El-Kassar N  et al. High levels of IL-7 cause dysregulation of thymocyte development. Int Immunol 24:661-71 (2012). Read more (PubMed: 22899673) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Hamster Cell (Dll-1 Transfected CHO)
Specification Dll-1 Transfected CHO
Fixative Formaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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Submitted May 07 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"