The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).
FunctionActs as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
Tissue specificityExpressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.
Anti-DLL1 antibody (ab85346) at 0.3 µg/ml + Rat Heart lysate at 35 µg
Predicted band size : 78 kDa Observed band size : 78 kDa
References for Anti-DLL1 antibody (ab85346)
This product has been referenced in:
Braune EB et al. S/T phosphorylation of DLL1 is required for full ligand activity in vitro but dispensable for DLL1 function in vivo during embryonic patterning and marginal zone B cell development. Mol Cell Biol34:1221-33 (2014).
Read more (PubMed: 24449764) »