Purification notesThe purity of ab110306 is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.25 µg/ml. Predicted molecular weight: 49 kDa.
Use a concentration of 1 µg/ml.
Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Use a concentration of 4 µg/ml. (0.4 µg/well)
Use at an assay dependent concentration.
FunctionThe 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).
PathwayAmino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.
Sequence similaritiesBelongs to the 2-oxoacid dehydrogenase family. Contains 1 lipoyl-binding domain.
Immunocytochemistry image of ab110306-stained Human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). Cells were then incubated with ab110306 at 1 µg/ml for 2 h at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
Flow Cytometry - OGDH antibody (ab110306)
HL-60 cells were stained with 1 µg/mL ab110306 (blue) or an equal amount of an isotype control antibody (red) and analyzed by Flow Cytometry.
Immunocytochemistry/ Immunofluorescence - Anti-DLST antibody [9F4BD5 ] (ab110306)This image is courtesy of an anonymous Abreview
ab110306 staining DLST in Human monocyte-derived macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 5% Goat serum for 60 minutes at 21°C. Samples were incubated with primary antibody (1/1000 in PBS + 1% BSA) for 12 hours at 4°C. A Cy2®-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody.
Flow Cytometry - Anti-DLST antibody [9F4BD5 ] (ab110306)This image is courtesy of an anonymous Abreview
ab110306 staining DLST in Human monocyte-derived macrophages by Flow Cytometry. Cells were prepared by scraoing in PBS and fixation by paraformaldehyde. The sample was incubated with the primary antibody (1/1000 in PBS + 1% BSA) for 60 minutes at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal(1/100) was used as the secondary antibody. Gating Strategy: Dead cells excluded.