![Immunocytochemistry/ Immunofluorescence - Anti-DLST antibody [9F4BD5 ] (ab110306) image](#OGDH-Primary-antibodies-ab110306-1.jpg)
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Product name
Anti-DLST antibody [9F4BD5 ]
See all DLST products (4) ...
Description
Mouse monoclonal [9F4BD5 ] to DLST
Tested applications
WB, ICC/IF, Flow Cyt, In-Cell ELISA, IPmore details
Cross reactivity
Reacts with
Cow, Human, Pig
Immunogen
Purified OGDH from Porcine heart.
Positive control
Human HDFn and HL-60 cells
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
The purity of ab110306 is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
9F4BD5
Isotype
IgG1
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Amino acid metabolism
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Energy Metabolism
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Hydrolysis
Signal Transduction >> Metabolism >> Amino Acids
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.25 µg/ml. Predicted molecular weight: 116 kDa.
ICC/IF: Use a concentration of 1 µg/ml.
Flow Cyt: Use a concentration of 1 µg/ml.
In-Cell ELISA: Use a concentration of 4 µg/ml. (0.4 µg/well)
IP: Use at an assay dependent dilution.
Target
Function
The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).
Pathway
Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.
Sequence similarities
Belongs to the 2-oxoacid dehydrogenase family.
Contains 1 lipoyl-binding domain.
Cellular localization
Mitochondrion.
Target information above from: UniProt accessionP36957
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Dihydrolipoyllysine residue succinyltransferase component of 2 oxoglutarate dehydrogenase complex antibody
- mitochondrial antibody
- 2-oxoglutarate dehydrogenase complex component E2 antibody
see all
Database links
- Entrez Gene: 1743 Human
- Omim: 126063 Human
- SwissProt: P36957 Human
- Unigene: 525459 Human
Anti-DLST antibody [9F4BD5 ] images:
Immunocytochemistry/ Immunofluorescence - Anti-DLST antibody [9F4BD5 ] (ab110306)
![Immunocytochemistry/ Immunofluorescence - Anti-DLST antibody [9F4BD5 ] (ab110306)](/ps/datasheet/images/110/ab110306/OGDH-Primary-antibodies-ab110306-1.jpg)
Immunocytochemistry image of ab110306-stained Human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). Cells were then incubated with ab110306 at 1 µg/ml for 2 h at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
Flow Cytometry - OGDH antibody (ab110306)
HL-60 cells were stained with 1 µg/mL ab110306 (blue) or an equal amount of an isotype control antibody (red) and analyzed by Flow Cytometry.
References for Anti-DLST antibody [9F4BD5 ] (ab110306)
ab110306 has not yet been referenced specifically in any publications.
Publishing research using ab110306? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"