Overview

  • Product name
    Anti-Dlx2 antibody [4B9]
    See all Dlx2 primary antibodies
  • Description
    Mouse monoclonal [4B9] to Dlx2
  • Tested applications
    Suitable for: ICC, ICC/IF, WB, Sandwich ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant Human Dlx2 protein (NP_004396.1).

  • Positive control
    • Human prostate tissue; NIH 3T3 cell lysate.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

Applications

Our Abpromise guarantee covers the use of ab117546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 34 kDa.
Sandwich ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Likely to play a regulatory role in the development of the ventral forebrain. May play a role in craniofacial patterning and morphogenesis.
  • Sequence similarities
    Belongs to the distal-less homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DII A antibody
    • Distal less homeo box 2 antibody
    • Distal less homeobox 2 antibody
    • DLX 2 antibody
    • Dlx2 antibody
    • DLX2_HUMAN antibody
    • Dlx5 antibody
    • Homeobox protein DLX 2 antibody
    • Homeobox protein DLX-2 antibody
    • Homeobox protein DLX2 antibody
    • Homeobox protein TES 1 antibody
    • Homeobox protein TES1 antibody
    • Tes 1 antibody
    • Tes1 antibody
    • zgc antibody
    see all

Images

  • ab117546, at 5 µg/ml, staining Dlx2 in Formalin-fixed, Paraffin-embedded Human Prostate tissue by Immunohistochemistry.
  • Anti-Dlx2 antibody [4B9] (ab117546) + NIH 3T3 cell lysate

    Predicted band size : 34 kDa
  • Detection limit for recombinant proprietary tagged Dlx2 is approximately 0.3 ng/ml as a capture antibody (ab117546).
  • Immunofluorescence analysis of murine adult NSCs derived from SVZ, staining Dlx2 with ab117546.

    Cells were fixed with Zamboni's fixative, permeabilized with 0.5% Triton X-100 and blocked with 3% normal goat serum for 2 hours at 20°C. Samples were incubated with primary antibody (1/500 in 3% normal goat serum) for 18 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Wang Y  et al. LSD1 co-repressor Rcor2 orchestrates neurogenesis in the developing mouse brain. Nat Commun 7:10481 (2016). IHC (PFA fixed) ; Mouse . Read more (PubMed: 26795843) »
  • Al-Jaberi N  et al. The Early Fetal Development of Human Neocortical GABAergic Interneurons. Cereb Cortex N/A:N/A (2013). IHC ; Human . Read more (PubMed: 24047602) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cultured Cells (adult NSC derived from SVZ (neurosphere))
Specification
adult NSC derived from SVZ (neurosphere)
Fixative
Zamboni's Fixative
Permeabilization
Yes - 0.5% TritonX-100 for 10min room temp
Blocking step
(agent) for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Dr. Michael Weible

Verified customer

Submitted Nov 07 2012

Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this antibody.

The details your customer has kindly provided...

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Expiration date: 2012-11-09


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