Overview

  • Product name
  • Description
    Rabbit polyclonal to Dlx2
  • Specificity
    ab30339 specifically detects Dlx2 in mouse and rat brain at 34 kDa. In our hands, ab30339 did not detect the same band in human brain (see data below).
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC (PFA fixed)
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide derived from within residues 200 - 300 of Mouse Dlx2.

    (Peptide available as ab19341.)

  • Positive control
    • This antibody gave a positive signal in mouse and rat embryonic and adult brain lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab30339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).Can be blocked with Mouse Dlx2 peptide (ab19341).
ICC/IF 1/200.
  • Application notes
    Is unsuitable for IHC (PFA fixed).
  • Target

    Images

    • All lanes : Anti-Dlx2 antibody (ab30339) at 1 µg/ml

      Lane 1 : E11 Embryo Brain (Mouse) Tissue Lysate
      Lane 2 : Mouse brain tissue lysate - total protein (0 days) (ab7188)
      Lane 3 : Brain (Mouse) Tissue Lysate
      Lane 4 : E14 Embryo Brain (Rat) Tissue Lysate
      Lane 5 : P0 Brain (Rat) Tissue Lysate
      Lane 6 : Brain (Rat) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 34 kDa
      Observed band size : 34 kDa


      Exposure time : 1 minute

      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab30339 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    • ab30339 staining Dlx2 in mouse Cor1 cells by ICC/IF. The cells were formaldehyde fixed, permeabilized in TBS/BSA/azide/Triton-x 0.3% buffer and blocked in 1% BSA for 30 minutes at 25°C. The primary antibody was diluted 1/200, and incubated with sample for 2 hour at 25°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG, diluted 1/600 was used as the secondary antibody.

      See Abreview

    • All lanes : Anti-Dlx2 antibody (ab30339) at 1/5000 dilution

      Lane 1 : Brain (Mouse) Tissue Lysate
      Lane 2 : Brain (Rat) Tissue Lysate
      Lane 3 : Human brain tissue lysate - total protein (ab29466)
      Lane 4 : Mouse brain tissue lysate - total protein (0 days) (ab7188)

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 34 kDa
      Observed band size : 34 kDa
      Additional bands at : 26 kDa,28 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 10 minutes

    References

    This product has been referenced in:
    • Wang Y  et al. A gain-of-function senescence bypass screen identifies the homeobox transcription factor DLX2 as a regulator of ATM-p53 signaling. Genes Dev 30:293-306 (2016). Read more (PubMed: 26833729) »
    • Bockstael O  et al. Rapid transgene expression in multiple precursor cell types of adult rat subventricular zone mediated by adeno-associated type 1 vectors. Hum Gene Ther 23:742-53 (2012). Read more (PubMed: 22471423) »

    See all 4 Publications for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cultured Cells (Inhibitory neurons derived from human iPSCs)
    Permeabilization
    Yes - 0.5% Triton-X 100
    Specification
    Inhibitory neurons derived from human iPSCs
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Apr 03 2017

    Application
    Flow Cytometry
    Sample
    Mouse Cell (Neural Stem Cells and Oligodendrocytes isolated fr)
    Permeabilization
    Yes - Ice cold methanol for 15 minutes
    Gating Strategy
    Prom1 (CD133) Positive neural stem and progenitors cells were sorted from the sub-ventricular zone of adult mice. Additionally, O4+ oligodendrocytes. Cells were negatively gated for cells from endothelial (CD31) and hematopoietic (CD45) lineages
    Specification
    Neural Stem Cells and Oligodendrocytes isolated fr
    Preparation
    Cell harvesting/tissue preparation method: The sub-ventricular zones of 3 adult C57Bl/6 mice were microdissected and dissociated using papain.
    Sample buffer: HBSS + 0.1% Glucose, 1% BSA
    Fixation
    Paraformaldehyde
    Username

    Ben

    Verified customer

    Submitted Mar 25 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Cor1 cells)
    Specification
    Cor1 cells
    Fixative
    Formaldehyde
    Permeabilization
    Yes - TBS/BSA/azide/Triton-x 0.3%
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Jun 05 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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