All lanes : Anti-Dlx2 antibody (ab30339) at 1 µg/ml
Lane 1 : E11 Embryo Brain (Mouse) Tissue Lysate Lane 2 :Mouse brain tissue lysate - total protein (0 days) (ab7188) Lane 3 : Brain (Mouse) Tissue Lysate Lane 4 : E14 Embryo Brain (Rat) Tissue Lysate Lane 5 : P0 Brain (Rat) Tissue Lysate Lane 6 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa Observed band size: 34 kDa
Exposure time: 1 minute
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab30339 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Immunocytochemistry/ Immunofluorescence - Dlx2 antibody - Neural Stem Cell Marker (ab30339)This image is courtesy of an Abreview submitted by Mr Carl Hobbs
ab30339 staining Dlx2 in mouse Cor1 cells by ICC/IF. The cells were formaldehyde fixed, permeabilized in TBS/BSA/azide/Triton-x 0.3% buffer and blocked in 1% BSA for 30 minutes at 25°C. The primary antibody was diluted 1/200, and incubated with sample for 2 hour at 25°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG, diluted 1/600 was used as the secondary antibody.
Wang Y et al. A gain-of-function senescence bypass screen identifies the homeobox transcription factor DLX2 as a regulator of ATM-p53 signaling. Genes Dev30:293-306 (2016).
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Bockstael O et al. Rapid transgene expression in multiple precursor cell types of adult rat subventricular zone mediated by adeno-associated type 1 vectors. Hum Gene Ther23:742-53 (2012).
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