Overview

  • Product nameAnti-Dlx2 antibody
    See all Dlx2 primary antibodies
  • Description
    Mouse monoclonal to Dlx2
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-110 of Human Dlx2

Properties

Applications

Our Abpromise guarantee covers the use of ab54725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 34 kDa.
IHC-P Use a concentration of 1 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionLikely to play a regulatory role in the development of the ventral forebrain. May play a role in craniofacial patterning and morphogenesis.
  • Sequence similaritiesBelongs to the distal-less homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DII A antibody
    • Distal less homeo box 2 antibody
    • Distal less homeobox 2 antibody
    • DLX 2 antibody
    • Dlx2 antibody
    • DLX2_HUMAN antibody
    • Dlx5 antibody
    • Homeobox protein DLX 2 antibody
    • Homeobox protein DLX-2 antibody
    • Homeobox protein DLX2 antibody
    • Homeobox protein TES 1 antibody
    • Homeobox protein TES1 antibody
    • Tes 1 antibody
    • Tes1 antibody
    • zgc antibody
    see all

Anti-Dlx2 antibody images

  • Dlx2 antibody (ab54725) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human lung.


  • Predicted band size : 34 kDa
    Dlx2 antibody (ab54725) at 1ug/lane + NIH/3T3 cell lysate at 25ug/lane.
  • Overlay histogram showing SH-SY5Y cells stained with ab54725 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54725, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Dlx2 antibody (ab54725)

ab54725 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"