Dlx2 antibody (ab54725) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human lung.
Western blot - Dlx2 antibody (ab54725)
Predicted band size : 34 kDa Dlx2 antibody (ab54725) at 1ug/lane + NIH/3T3 cell lysate at 25ug/lane.
Flow Cytometry - Anti-Dlx2 antibody (ab54725)
Overlay histogram showing SH-SY5Y cells stained with ab54725 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54725, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-Dlx2 antibody (ab54725)
has not yet been referenced specifically in any publications.
Publishing research using ab54725? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"