The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Detection limit for recombinant tagged Dlx5 is approximately 0.03ng/ml if used as a capture antibody.
1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa). This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.