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Product name Anti-Dlx5 antibody See all Dlx5 primary antibodies
Rabbit polyclonal to Dlx5
Specificity ab64827 detects endogenous levels of total Dlx5 protein.
Tested applications Suitable for:
ELISA, IHC-P, WB, IHC-Fr more details
Mouse, Rat, Human
Synthetic peptide derived from an internal sequence of human Dlx5.
Human lung carcinoma tissue and extracts from HeLa, 293 and COLO205 cells.
Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab64827 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes ELISA: 1/40000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Cellular localization Nuclear
Distal less homeo box 5 antibody
Distal less homeobox 5 antibody
Dlx 5 antibody
Anti-Dlx5 antibody images
Western blot - Dlx5 antibody (ab64827)
All lanes : Anti-Dlx5 antibody (ab64827) at 1/500 dilution Lane 1 : HeLa cell extract at 30 µg Lane 2 : 293 cell extract at 30 µg Lane 3 : COLO205 cell extract at 30 µg Lane 4 : COLO205 cell extract at 30 µg
plus immunising peptide at 10 µg Predicted band size : 32 kDa Observed band size : 32 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Dlx5 antibody (ab64827)
ab64827, at 1/50 dilution, staining Dlx5 in paraffin embedded human lung carcinoma tissue by Immunohistochemistry in the absence or presence of the immunising peptide.
Immunohistochemistry (Frozen sections) - Dlx5 antibody (ab64827) This image is courtesy of an anonymous Abreview
ab64827 at 1/100 dilution staining Dlx5 in mouse nasal bone anlagen by immunohistochemistry (frozen sections). Sections were paraformaldehyde fixed, permeabilized in 0.1% prior to blocking in 20% serum for 1 hour at RT and then incubated with ab64827 for 16 hours at 4°C. Alexa fluor® 488 donkey polyclonal, diluted 1/200, was used as the secondary antibody.
References for Anti-Dlx5 antibody (ab64827)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"