Anti-DMAP1 antibody (ab3737)

Overview

  • Product nameAnti-DMAP1 antibody
    See all DMAP1 primary antibodies
  • Description
    Goat polyclonal to DMAP1
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide (Human) - which represented a portion of human DNA methyltransferase 1-associated protein 1 encoded within exon 8.

  • Positive control
    • HeLa cell lysate.
  • General notes


    Involved in transcription repression. Its interaction with HDAC2 may provide a platform for how histones may become deacetylated in heterochromatin following replication. Can repress transcription independently of histone deacetylase activity.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesInvolved in transcription repression. Its interaction with HDAC2 may provide a platform for how histones may become deacetylated in heterochromatin following replication. Can repress transcription independently of histone deacetylase activity.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
WB 1/1000 - 1/5000. Predicted molecular weight: 65 kDa.

Target

  • RelevanceDMAP1 is involved in transcription repression and activation. Its interaction with HDAC2 may provide a mechanism for histone deacetylation in heterochromatin following replication of DNA at late firing origins. Can also repress transcription independently of histone deacetylase activity. May specifically potentiate DAXX-mediated repression of glucocorticoid receptor-dependent transcription. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • DKFZp686L09142 antibody
    • DMAP 1 antibody
    • DNA methyltransferase 1 associated protein 1 antibody
    • DNMAP 1 antibody
    • DNMAP1 antibody
    • DNMT1 associated protein 1 antibody
    • DNMTAP 1 antibody
    • DNMTAP1 antibody
    • EAF 2 antibody
    • EAF2 antibody
    • FLJ11543 antibody
    • KIAA1425 antibody
    • MAT 1 mediated transcriptional repressor antibody
    • MGC55593 antibody
    • SWC 4 antibody
    • SWC4 antibody
    • zgc:55593 antibody
    see all

Anti-DMAP1 antibody images



  • Predicted band size : 65 kDa


    Sample: Hela cell lysate (50µg/lane)

    Antibody: Affinity purified Goat anti-DMAP 1 (ab3737) used at the concentrations indicated.

    Detection: ECL exposed for 2 minutes.

  • ab3737 (1/200) staining DMAP1 in assynchronous HeLa cells. Cells were fixed in paraformaldehyde and counterstained with DAPI in order to highlight the nucleus. Please refer to abreview for further experimental details.

    See Abreview

References for Anti-DMAP1 antibody (ab3737)

ab3737 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

Submitted Jun 27 2007

Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab3737. The exact Western blotting protocol that was used to characterize this antibody is not available but I would like to make the following suggestions to he...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"