Anti-DMAP1 antibody (ab3737)

Overview

  • Product name
  • Description
    Goat polyclonal to DMAP1
  • Host species
    Goat
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide (Human) - which represented a portion of human DNA methyltransferase 1-associated protein 1 encoded within exon 8.

  • Positive control
    • HeLa cell lysate.
  • General notes


    Involved in transcription repression. Its interaction with HDAC2 may provide a platform for how histones may become deacetylated in heterochromatin following replication. Can repress transcription independently of histone deacetylase activity.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Involved in transcription repression. Its interaction with HDAC2 may provide a platform for how histones may become deacetylated in heterochromatin following replication. Can repress transcription independently of histone deacetylase activity.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
WB 1/1000 - 1/5000. Predicted molecular weight: 65 kDa.

Target

  • Relevance
    DMAP1 is involved in transcription repression and activation. Its interaction with HDAC2 may provide a mechanism for histone deacetylation in heterochromatin following replication of DNA at late firing origins. Can also repress transcription independently of histone deacetylase activity. May specifically potentiate DAXX-mediated repression of glucocorticoid receptor-dependent transcription. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • DKFZp686L09142 antibody
    • DMAP 1 antibody
    • DNA methyltransferase 1 associated protein 1 antibody
    • DNMAP 1 antibody
    • DNMAP1 antibody
    • DNMT1 associated protein 1 antibody
    • DNMTAP 1 antibody
    • DNMTAP1 antibody
    • EAF 2 antibody
    • EAF2 antibody
    • FLJ11543 antibody
    • KIAA1425 antibody
    • MAT 1 mediated transcriptional repressor antibody
    • MGC55593 antibody
    • SWC 4 antibody
    • SWC4 antibody
    • zgc:55593 antibody
    see all

Images

  • Sample: Hela cell lysate (50µg/lane)

    Antibody: Affinity purified Goat anti-DMAP 1 (ab3737) used at the concentrations indicated.

    Detection: ECL exposed for 2 minutes.

     

    Sample: Hela cell lysate (50µg/lane)

    Antibody: Affinity purified Goat anti-DMAP 1 (ab3737) used at the concentrations indicated.

    Detection: ECL exposed for 2 minutes.

  • ab3737 (1/200) staining DMAP1 in assynchronous HeLa cells. Cells were fixed in paraformaldehyde and counterstained with DAPI in order to highlight the nucleus. Please refer to abreview for further experimental details.

    See Abreview

References

ab3737 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Jun 27 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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