Recombinant
RabMAb

Anti-DMAP1 antibody [EPR18179] (ab188407)

Overview

  • Product name
    Anti-DMAP1 antibody [EPR18179]
    See all DMAP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR18179] to DMAP1
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DMAP1 aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9NPF5

  • Positive control
    • WB: HeLa, Jurkat, 293, MCF7, Ramos, Neuro-2a, Raw264.7, PC12, NIH 3T3 cell lysates; HeLa nuclear fraction lysate. ICC/IF: HeLa and F9 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab188407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/2000.
WB 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

Target

  • Relevance
    DMAP1 is involved in transcription repression and activation. Its interaction with HDAC2 may provide a mechanism for histone deacetylation in heterochromatin following replication of DNA at late firing origins. Can also repress transcription independently of histone deacetylase activity. May specifically potentiate DAXX-mediated repression of glucocorticoid receptor-dependent transcription. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • DKFZp686L09142 antibody
    • DMAP 1 antibody
    • DNA methyltransferase 1 associated protein 1 antibody
    • DNMAP 1 antibody
    • DNMAP1 antibody
    • DNMT1 associated protein 1 antibody
    • DNMTAP 1 antibody
    • DNMTAP1 antibody
    • EAF 2 antibody
    • EAF2 antibody
    • FLJ11543 antibody
    • KIAA1425 antibody
    • MAT 1 mediated transcriptional repressor antibody
    • MGC55593 antibody
    • SWC 4 antibody
    • SWC4 antibody
    • zgc:55593 antibody
    see all

Images

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling DMAP1 with ab188407 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear staining on HeLa cell line.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
    The negative controls are as follows:
    1. ab188407 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

     

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized F9 (Mouse embryonic carcinoma cell line) cells labeling DMAP1 with ab188407 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear staining on F9 cell line.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
    The negative controls are as follows:
    1. ab188407 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

     

  • All lanes : Anti-DMAP1 antibody [EPR18179] (ab188407) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 3 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 5 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
    Lane 6 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 53 kDa
    Observed band size : 53 kDa


    Exposure time : 3 minutes

    5% NFDM/TBST: Blocking and diluting buffer.

  • All lanes : Anti-DMAP1 antibody [EPR18179] (ab188407) at 1/1000 dilution

    Lane 1 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : PC12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 3 : NIH 3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 53 kDa
    Observed band size : 53 kDa


    Exposure time : 3 minutes

    5% NFDM/TBST: Blocking and diluting buffer.

  • All lanes : Anti-DMAP1 antibody [EPR18179] (ab188407) at 1/2000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cytosolic fraction
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) nuclear fraction

    Lysates/proteins at 10 µg per lane.

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 53 kDa
    Observed band size : 53 kDa


    Exposure time : 3 minutes

    Blocking and dilution buffer: 5% NFDM/TBST.

References

ab188407 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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