Overview

  • Product nameAnti-DMC1 antibody
    See all DMC1 primary antibodies
  • Description
    Mouse monoclonal to DMC1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 237-339 of Human DMC1 (NP_008999) with a proprietary tag.

  • Positive control
    • Tagged recombinant Human DMC1 fragment (the immunogen), PC-12 cell lysate and Hela nuclear extract.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab88426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 38 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionMay participate in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks.
  • Sequence similaritiesBelongs to the RecA family. DMC1 subfamily.
    Contains 1 HhH domain.
  • Cellular localizationNucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Disrupted meiotic cDNA 1 homolog antibody
    • Disrupted meiotic cDNA 1, yeast, homolog of antibody
    • dJ199H16.1 antibody
    • DMC 1 antibody
    • DMC1 antibody
    • DMC1 dosage suppressor of mck1 homolog antibody
    • DMC1 dosage suppressor of mck1 homolog meiosis specific homologous recombination (yeast) antibody
    • DMC1 homologue antibody
    • DMC1_HUMAN antibody
    • DMC1H antibody
    • DNA meiotic recombinase 1 antibody
    • HsLim15 antibody
    • LIM15 antibody
    • Meiotic recombination protein DMC1/LIM15 homolog antibody
    • MGC150472 antibody
    • MGC150473 antibody
    see all

Anti-DMC1 antibody images

  • Anti-DMC1 antibody (ab88426) at 1 µg/ml + Tagged recombinant Human DMC1 fragment (the immunogen) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 38 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-DMC1 antibody (ab88426) at 1 µg/ml + PC-12 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Additional bands at : 44 kDa,50 kDa. We are unsure as to the identity of these extra bands.
  • Anti-DMC1 antibody (ab88426) at 1 µg/ml + Hela nuclear extract at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 38 kDa
    Observed band size : 38 kDa

References for Anti-DMC1 antibody (ab88426)

ab88426 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"