Overview

  • Product name
  • Description
    Rabbit polyclonal to DMC1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, RatDoes not react with: Arabidopsis thaliana
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 and 176 of Human DMC1 (NP_008999).

  • Positive control
    • WB: 293T, HeLa or Raji cell lysate, MOLT4 whole cell lysate; IHC-P: Cal27 xenograft.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 38 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function
    May participate in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks.
  • Sequence similarities
    Belongs to the RecA family. DMC1 subfamily.
    Contains 1 HhH domain.
  • Cellular localization
    Nucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Disrupted meiotic cDNA 1 homolog antibody
    • Disrupted meiotic cDNA 1, yeast, homolog of antibody
    • dJ199H16.1 antibody
    • DMC 1 antibody
    • DMC1 antibody
    • DMC1 dosage suppressor of mck1 homolog antibody
    • DMC1 dosage suppressor of mck1 homolog meiosis specific homologous recombination (yeast) antibody
    • DMC1 homologue antibody
    • DMC1_HUMAN antibody
    • DMC1H antibody
    • DNA meiotic recombinase 1 antibody
    • HsLim15 antibody
    • LIM15 antibody
    • Meiotic recombination protein DMC1/LIM15 homolog antibody
    • MGC150472 antibody
    • MGC150473 antibody
    see all

Images

  • Anti-DMC1 antibody (ab96613) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size : 38 kDa
  • Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using ab96613 at 1/500 dilution.

References

This product has been referenced in:
  • White YA  et al. Oocyte formation by mitotically active germ cells purified from ovaries of reproductive-age women. Nat Med 18:413-21 (2012). Read more (PubMed: 22366948) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
900 µg
Gel Running Conditions
Reduced Denaturing (12%, Laemmli)
Sample
Arabidopsis thaliana Tissue lysate - whole (closed flower buds)
Specification
closed flower buds
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Natasha Elina

Verified customer

Submitted Mar 04 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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