Overview

  • Product name
    Anti-DmIh antibody
  • Description
    Mouse polyclonal to DmIh
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster
  • Immunogen

    Vector coding for a partial recombinant fusion protein, corresponding to C terminal amino acids 1252-1327 of Fruit fly (Drosophila melanogaster) DmIh. HQKDEQLSNP ESNTITAVVN ALAAEADDCK DDDMDLKENL LHGSESSIA EPVQTIREGL PRPRSGEFRA LFEGNTP.

  • General notes

     

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: None
    Constituents: 50% Glycerol, Whole serum
  • Purity
    Whole antiserum
  • Primary antibody notes
    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 147 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

  • Relevance
    DmIh is an intracellular cyclic nucleotide activated cation channel.
  • Cellular localization
    Plasma membrane
  • Database links
    • Alternative names
      • DMIH antibody
      • HCN4 antibody
      • Ih antibody
      • Putative Ih channel antibody
      • Putative voltage and cyclic nucleotide gated ion channel antibody
      see all

    Images

    • All lanes : Anti-DmIh antibody (ab52694) at 1/1000 dilution

      Lane 1 : Total protein extract from E. coli containing 50 - 100ng of a tagged fusion protein of an irrelevant antigen
      Lane 2 : Total protein extract from E. coli containing 50 - 500ng of the antigen (tagged fusion protein)

      Lysates/proteins at 20 µg per lane.

      Secondary
      Rabbit anti-mouse IgG + IgM HRP conjugated, at 1/5000 dilution

      Predicted band size : 147 kDa

    References

    ab52694 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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